Biochem lecture 32

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swynocker
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47801
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Biochem lecture 32
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2010-11-06 22:32:38
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biochem lecture
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Biochem 32 ex3
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  1. Transport of ammonia to the liver is mostly in the form of _____ or ______.
    Glutamine or alanine
  2. _____ ______ is responsible for te synthesis of glutamine from glutamate and NH3
    Glutamine synthetase.
  3. glutamate is synthesized by the _____ amination of __________ catalyzed by glutamate dehydrogenase.
    reductive, a-ketoglutarate
  4. Glutamate arises from __________ reactions with the amino nitrogen being donated by a number of different _____ _____.
    • aminotransferase
    • amino acids
  5. Glutamine is produced from glutamate by the direct incorporation of _______; which can be considered a ____ _____ reaction.
    • Ammonia
    • nitrogen fixing
  6. Role of glutamine is to carry ammonia from ______ _____ to the ____ where it is converted to ammonia by ________.
    • Peripheral tissues to the liver
    • glutaminase
  7. Second mechanism of ammonia transport is by
    alanine
  8. pyruvate is transaminated to form _____
    alanine
  9. Synthesis of urea requires 5 enzymes
    • Carbamoyl phosphate synthetase I
    • orithinine transcarbomoylase
    • argininosuccinate synthetase
    • agrininosuccinate lyase
    • arginase
  10. Carbomoyl phosphate is the ______ and is inside the ______.
    • Rate limiting step
    • mitochondria
  11. arginine is an allosteric _____
    activator
  12. There are _#_ ATPs used in the urea cycle.
    4
  13. OTC is for the formation of
    Citrulline
  14. Oxaloacetate fates
    • Transamination to aspartate
    • Conversion into glucose by gluconeogenic path
    • condensation with acetly-CoA to form citrate
    • Conversion to pyruvate
  15. Amino group of amino acids are transferred to ________, forming an alpha-keto acid and glutamate via ______.
    • a-ketoglutarate
    • transaminase.
  16. Glutamate is deaminated to form _______ and to regenerate _____ via glutamine dehydrogenase.
    Ammonia and to regenerate a-ketoglutarate
  17. Conversion and elimination of ammonia as _____ via ______ _____ _________ and the urea cycle enzymes.
    • Urea
    • Carbomyl Phosphate synthetase
  18. Metabolism of the carbon backbone occurs via the
    TCA cycle

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