Microbiology iClicker Questions Exam 2

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shutterbug81
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Microbiology iClicker Questions Exam 2
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2010-11-07 14:26:52
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Microbiology
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Microbiology
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  1. If asked to sterilize a heat sensitive material, which would you use:
    A. steam autoclave
    B. ethylene oxide
    C. hot air
    D. pasteurization
    B. ethylene oxide
    (this multiple choice question has been scrambled)
  2. Which is incorrect:
    A. biofilms protect bacteria
    B. biofilms make bacteria more sensitive to antibiotics
    C. biofilms attach bacteria to surfaces
    D. biofilms are often polymicrobial
    B. biofilms make bacteria more sensitive to antibiotics
    (this multiple choice question has been scrambled)
  3. Which is not a chemical agent used to control microbes:
    A. halogens
    B. gluteraldehyde
    C. filtration
    D. phenol
    C. filtration
    (this multiple choice question has been scrambled)
  4. Household bleach is used as common disinfectant because it has:
    A. hypochlorite
    B. iodine
    C. phenol
    D. cyclohexidine
    A. hypochlorite
    (this multiple choice question has been scrambled)
  5. Resistance to antibiotics can result from the following mechanisms, except:
    A. inactivation of the drug
    B. overproduction of targeted enzyme
    C. underproduction of membrane components
    C. underproduction of membrane components
    (this multiple choice question has been scrambled)
  6. Which has the highest energy:
    A. phosphate ether bonds
    B. phosphate ester bonds
    C. thioester bonds
    D. phosphate anhydride bonds
    D. phosphate anhydride bonds
    (this multiple choice question has been scrambled)
  7. All energy in a living system is derived from
    - movement of electrons down an energy gradient
  8. If G˚’<0 then:
    - the reaction is exergonic and can occur spontaneously
  9. If organism A uses H2S as an electon donor and Fe3+ as electron acceptor AND organism B uses methanol as electron donor and O2 as electron acceptor, which probably produces the most energy:
    - Organism B (because O2 is electron acceptor)
  10. During glycolysis: R-P +ADP à ATP + R. What is this an example of:
    - substrate level phosphorylation
  11. What is the main purpose of fermentation?
    - Regeneration of NAD+
  12. What is the purpose of the Kreb’s Cycle?
    A. Make intermediates for biosynthesis reactions
    B. Form molecules to shuttle electrons to ETC
    C. ALL
    D. Make ATP
    E. Completely oxidize pyruvate
    C. ALL
    (this multiple choice question has been scrambled)
  13. If a cell is not working properly and can’t convert pyruvate to acetyl CoA, what would be the total ATP production?
    2 ATP
  14. Which is produced in the Kreb’s Cycle and used for biosynthesis of amino acids?
    A. Ammonia
    B. Ketogluterate
    C. Glutamate
    D. Glutamine
    B. Ketogluterate
    (this multiple choice question has been scrambled)
  15. Which of these are carried by NADH and FADH2 and ultimately lead to ATP in the ETC?
    A. hydrogen ions
    B. electrons
    C. electrons & hydrogen ions
    D. acetyl CoA
    E. phosphorous
    C. electrons & hydrogen ions
    (this multiple choice question has been scrambled)
  16. Which is true for NADH?
    Has most negative Eo and greatest capacity to donate electrons.
  17. Place in the correct order that electrons are transferred:
    - NADH, FMN, Quinone, Cyt C, Cyt A, O2
  18. Which electron carriers associated with electron transport are lipid soluble?
    - Quinones
  19. When glucose is used as an energy source and O2 is the final electron acceptor, how many ATP are formed:
    A. 20
    B. 4
    C. 38
    D. 2
    E. 16
    C. 38
    (this multiple choice question has been scrambled)
  20. How is ATP produced?
    - Harvesting the transmembrane gradient by ATP-synthase
  21. Oxidation reductase pairs X/XH2 and Y/YH2 have reduction potentials of -50mV and +75mV. This means an electron would most likely be removed from ______ to reduce _____.
    XH2, Y
  22. Aerobic respiration differs from anaerobic respiration in which respect?
    - The final electron acceptors are different
  23. A bacteria converts nitrate to nitrogen gas. This process is called:
    A. fermentation
    B. nitrification
    C. denitrification
    D. nitrogen fixation
    C. denitrification
    (this multiple choice question has been scrambled)
  24. Which microbes use CO2 as an electron acceptor?
    A. a & b
    B. sulfate reducing bacteria
    C. homoacetogens
    D. methanogens
    e. all of the above
    A. a & b
    (this multiple choice question has been scrambled)
  25. Which is not a final electron acceptor during anaerobic respiration?
    A. chlorate
    B. ferric ion
    C. succinate
    D. manganic ion
    E. arsenate
    C. succinate
    (this multiple choice question has been scrambled)
  26. Place these in order of highest energy yield to lowest:
    1. Respiration with O2 as electron acceptor
    2. Respiration with nitrate as electron acceptor
    3. Respiration with ferric ion as electron acceptor
    4. Catabolism of substrate linked to ion transport
    • 1. Respiration with O2 as electron acceptor
    • 2. Respiration with nitrate as electron acceptor
    • 3. Respiration with ferric ion as electron acceptor
    • 4. Catabolism of substrate linked to ion transport
  27. Which is incorrect for anoxygenic photosynthesis?
    A. The electron donor is H20
    B. photopigments are chlorophylls
    C. There is one photosystem (PSI)
    D. electron donor is hydrogen sulfide, H2, or sulfur
    A. The electron donor is H20
    (this multiple choice question has been scrambled)
  28. The enzyme hydroxylamine oxidoreductase…
    - oxidizes NH2OH to NO2
  29. The enzyme nitrite oxidoreductase…
    - oxidizes NO2 to NO3
  30. In chemolithotrophic bacteria that use H2 as an electron donor, which is incorrect:
    - acetic acid is produced at the terminal oxidase
  31. In the nitrogen cycle, the final product of _____:
    A. nitrogen fixation is ammonia
    B. nitrification is ammonia
    C. nitrification is nitrate
    D. denitrification is nitrogen gas
    B. nitrification is ammonia
    (this multiple choice question has been scrambled)
  32. In autotrophic bacteria, CO2 is fixed by:
    A. reverse TCA cycle
    B. a&b
    C. calvin cycle
    D. a,b, &c
    E. hydroxypropinate cycle
    D. a,b, &c
    (this multiple choice question has been scrambled)
  33. Select the correct statements about nitrogenase:
    1. Nitrogenase-reduces cyanide, acetylene, and other triple bonded compounds
    2. Nitrogenase is transcriptionally regulated by oxygen, ammonia, and certain amino acids
    3. Nitrogenase catalyzes the denitrification of ammonia
    4. Nitrogenase is the terminal oxidase in anaerobic respiration
    1 & 2
  34. You dilute samples before plating in enrichment because:
    - you want to dilute out weed species that grow quickly but aren’t that abundant
  35. To eliminate the live-to-dead ratio of cells in sample, use:
    A. DAPI
    B. Bac Light
    C. GFP
    B. Bac Light
    (this multiple choice question has been scrambled)
  36. Why are killed cell controls so important when using radioisotopes to measure microbial transformations?
    A. Because some transformations can occur abiotically and controls will help you asses those abiotic transformations
    B. It will let you know if the transformations are occurring in the reverse direction
    C. They can help you determine the rate of transformation
    D. It lets you know how many organisms die during the transformation
    A. Because some transformations can occur abiotically and controls will help you asses those abiotic transformations
    (this multiple choice question has been scrambled)
  37. Organism A converts cellulose to cellobiose. Organism B converts cellobiose to glucose. What is their relationship?
    -Sytrophic
  38. Which of the following is not mutualism?
    A. termites and bacteria in their gut
    B. lichens
    C. Beggiatoa oxidation of H2O2
    D. legumes and sinorhizobia interaction
    C. Beggiatoa oxidation of H2O2
    (this multiple choice question has been scrambled)
  39. Each band on a DGGE gel represents ____:
    A. A different DNA fragment produced after treating the DNA with restriction enzymes
    B. A different phylotype/strain represented in the community DNA
    C. A different community of DNA
    D. A different PCR product from the PCR amplification of the total community DNA
    B. A different phylotype/strain represented in the community DNA
    (this multiple choice question has been scrambled)
  40. If you have a group of various species of microorganisms that can all oxidize sulfide in a given habitat, this is called a _____
    A. population
    B. ecosystem
    C. guild
    D. community
    C. guild
    (this multiple choice question has been scrambled)
  41. Where is microbe activity highest in soil?
    A. The surface soil
    B. The B horizon
    C. The O horizon
    D. The rhizosphere
    D. The rhizosphere
    (this multiple choice question has been scrambled)
  42. Select the incorrect statement about biofilms.
    A. biofilms cause dental plaque
    B. biofilms are likely to be resistant to antimicrobial agents
    C. biofilms are protected from attack by immune system
    D. biofilms only cause medically related problems
    D. biofilms only cause medically related problems
    (this multiple choice question has been scrambled)
  43. In one common symbiotic relationship one of the associates benefits from association. The other is either harmed or even killed. This form of symbiosis is:
    A. parasitic
    B. common symbiosis
    C. commensalistic
    D. mutualistic
    A. parasitic
    (this multiple choice question has been scrambled)
  44. Which of the following would be a reason for the observation that generally microbes grow more slowly in nature than they do in laboratory culture?
    a. Microbes in nature rarely exist in pure culture, and must compete for resources
    b. Nutrients are frequently in low supply in nature
    c. The distribution of nutrients is rarely uniform throughout an environment
    d. All of the above
    e. More than one of the above, but not all
    d. All of the above
  45. A microbe that lives at a depth of 5000m in the ocean would be
    a. Halophilic
    b. Barophilic
    c. Psychrophilic
    d. Barotolerant
    e. more than one of the above, but not all
    e. more than one of the above, but not all
  46. Which of the following is an incorrect statement about pelagic and benthic microbes?
    A. Benthic microbes are found at the bottom of the sea
    B. Pelagic microbes are usually attached to particulate matter
    C. Most benthic microbes are phototrophs
    D. Pelagic microbes are found in the water column
    C. Most benthic microbes are phototrophs
    (this multiple choice question has been scrambled)
  47. Choose the correct statement(s) about endoliths.
    A. use as their energy supply hydrogen gas from uranium decay which generates hydrogen radicals
    B. a,b,&c
    C. are autotrophs since they obtain carbon from carbohydrates in the rock
    D. are bacteria growing within the crystals of solid rock
    E. a&b
    B. a,b,&c
    (this multiple choice question has been scrambled)
  48. The use of microbes to degrade and detoxify pollutants is known as
    A. flocculation
    B. bioremediation
    C. eutrophication
    D. primary sewage treatment
    B. bioremediation
    (this multiple choice question has been scrambled)
  49. In competition for nutrients and resources, bacteria may secrete bacteriocins which are:
    A. peptides that disrupt the cell membrane
    B. peptides that disrupt the proton gradient
    C. antibiotics that affect the cell wall
    D. antibiotics that inhibit protein synthesis
    A. peptides that disrupt the cell membrane
    (this multiple choice question has been scrambled)
  50. Which of the following is not difference between prokaryotes and eukaryotes?
    A. presence of nucleoid in eukaryotes
    B. processing of RNA before translation in eukaryotes
    C. presence of operons coding for polycistronic RNAs in prokaryotes
    D. presence of introns in eukaryotes
    A. presence of nucleoid in eukaryotes
    (this multiple choice question has been scrambled)

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