BIOCHEM Molecular techniques

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BIOCHEM Molecular techniques
2010-11-07 15:39:27

Molecular Techniques; Griffith BIOCHEM
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  1. Restriction enzymes
    are bacterial proteins that cleave DNA at specific sequences.
    is a palindrome and a restriction site.
  3. List the importance of restriction enzymes in the treatment of type I & II diabetes.
    • The human insulin gene is removed from human DNA and is subcloned into a bacterial plasmid.
    • The bacteria then produce human insulin which can be used to treat type I & II diabetes.
  4. DNA is negatively charged.
  5. DNA is separated on gel electrophoresis based on:
    molecular weight.
  6. Southern Blot
    is used to identify DNA.
  7. Northern Blot
    is used to identify RNA.
  8. Western Blot
    is used to identify protein.
  9. A probe
    • is defined as a single-stranded piece of DNA or RNA that is used to visualize complementary
    • sequences within DNA.
  10. DNA sequencing
    utilizes dideoxy nucleotides to sequence segments of DNA.
  11. PCR
    stands for polymerase chain reaction and is a technique used to amplify DNA.
  12. Taq polymerase
    is derived from the bacteria Thermus Aquaticus commonly found in hot springs.
  13. A gene knockout
    is a technique used by researchers to knockout a particular gene of interest.
  14. A karyotype
    • is a technique used to identify DNA chromosomal abnormalities. For example, trisomies,
    • monosomies, and various other abnormalities can be detected by karyotypes.
  15. Cloning
    is a technique used to create an exact copy of oneself.
  16. RNAi
    • inhibitor RNAs
    • is used to inhibit protein expression by inducing the degradation of mRNA.
  17. Dicer
    is the enzyme responsible for cleavage of RNAi into siRNA.
  18. RISC
    • stands for RNA induced silencing complex and is the enzyme responsible for cleaving the
    • endogenous mRNA.
  19. List the diseases currently being investigated for RNAi treatment.
    • Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential
    • diseases being investigated as potential RNAi targets.