ch 9

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restriction enzumes were first discovered with the observation that

phage DNA is destroyed in a host cell
the DNA probe, 3'-GGCTTA, will hybridize with which of the following?

5'-CCGAAT
which of the following is the fourth basic step to genetically modify a cell?

ligation
the following enzymes are used to make cDNA, what is the second enzyme used to make cDNA

ribozyme
if you put a gene in a virus, the next step in genetic modification would be

transduction
you have a small gene that you want replicated by PCR. you add radioactively labeled nucleotides to the PCR thermalcycler. after three replication cycles what percentage of DNA single-strands are radioactively labeled?

87.5%
pieces of human DNA stored in yeast cells

library
a population of cells carrying a desired plasmid

clone
self replicating DNA for transmitting a gene from one organism to another

vector
a gene that hybridizes with mRNA

antisense