Card Set Information
Why are enzymes complex?
Speed up reaction by factor of millions
Reduce energy of activation
What is the Transition State?
A state of high energy level in reaction leading to product formation.
Will an enzyme work outside of it normal temperature or pH range? Why?
It is denatured
What is the equation for equilibrium constant?
Keq = KF[A]n/KR[B]m = [B]m/[A]n
What happens at the active site of an enzyme?
Holoenzyme - Subrate banding and catalytic activity
What are the two parts of an enzyme?
Non-protein - cofactor
What is the lock and key model of an enzyme?
Doesn't take into account protein conformations.
What is the induced-fit model of action enzyme?
Assumes enzyme conformation changes to accommodate the substrate molecule
How are enzymes classified?
According to the type of chemical reaction is catalyzes.
What are the six enzyme categories?
What is kinetics?
The rate (velocity) and mechanism of a reaction
How are rates usually measured?
How many moles of reactant or product are changed per time period.
What is a mechanism?
A detailed step by step description of how a reaction occurs at the molecular level.
How are all rate equations determined?
When the addition of more reactant has no effect on the rate what is it called?
What is a competitive inhibitor?
Looks like the substrate and binds to enzyme at active site
What is an uncompetative inhibitor?
Binds only to the enzyme-substrate complex
What is a noncompetitive inhibitor?
Doesn't look like the substrate
Doesn't bind at the active site
What do allosteric enzymes show?
Why are transition metals useful as catalysis?
High positive charge
Accepts electrons (Lewis acid)
Mediate redox reaction
Polarize water molecules
Coenzymes are organic molecules often derived from what?
What is the mechanism for Chymtrypsin?
Catalyzes the hydrolysis of peptide bonds next to aromatic side chains.
What are some methods that organisms use to regulate enzyme activity?
What is an example of enzyme induction?
E. coli is induced to use lactose in the absence of glucose.
Turned on by genetic control
When the product of a biochemical pathway inhibits the functioning of a key enzyme of a previous step in the pathway it is called _______ _______?
What are the two allosteric models?
What does the term carbohydrate mean?
Compounds associated with polyhydroxy
What are the names for monosaccarides with 3, 4, 5, and 6 carbons?
3 - triose
4 - tetrose
5 - pentose
6 - hexose
What are enantiomers?
Non-superimposable mirror image molecules
Where are most of the oxidized carbon in a Fischer projection?
At the top
What are anomers?
The alpha and beta forms of cyclic sugars
What is cellulose?
The major structural polymer in plants
What are starches?
Storage forms of glucose in plants
What is glycogen?
The storage carbohydrate in animals (liver and muscle cells)
What is chitin?
Provides structural support for exoskeleton in invertebrates.
What is a glycoconjugate?
Compounds that convalently link carbs to proteins and lipids
What is glycolysis?
Break down of an organic molecule (sugar) to make energy
What is the net ATP formed from glycolysis?
What is pyruvate?
a 3 carbon molecule
What is gluconeogenesis?
Making glucose from noncarbohydrate sources. ( 3 irreversible steps of glycolysis are bypassed)
What is is called when lactate from the skeletal muscle is transferred to the liver where it is converted to pyruvate then glucose?
What is is called when pyruvate in the muscles is converted to alanine which is transported to the liven and reconverted to pyruvate?
What happens in the pentose phosphate pathway?
Five Carbon Sugars are produced
NADPH is produced for biosynthesis
Reshuffling of carbons to give products with 3,4, 6,7 carbons
If NADPH is need, what steps must occur?
What reacts via the pentose phosphate pathway t make ribose?
Fructose and glyceraldhyde
What removes glucose units until 4 are left approaching a branch point?
What is central to the control of glycogen metabolism and is overall an important means of enzyme regulation?