-Nuclear Coarse Speckled- MCTD, SLE, Sjogrens syndrome (antigen U1-snRNP or Sm proteins)
-Nucleolar- 25-50% of patients with myositis-scleroderma (PM-Scl complex antigen)
-Cell Cycle- Crest Syndrome (antigen is kinetochore plates)
-Cytoplasmic- Primary biliary cirrhosis (antigen is mitochondrial autoantigen M2)
What is a diagnostic Marker?
Marker, blood: A sign of a disease or condition that can be isolated from a blood sample. For example, the monoclonal antibody D8/17 is a diagnostic sign of Pediatric Autoimmune Disorders associated with Strep
dsDNA (Double Stranded DNA) is?
1) a Diagnostic marker for systemic Lupus
2) Autoantigen is the nucleic acid DNA (of patient) against which antibodies are produced.
3) not present in healthy individuals.
dsDNA detection methods?
1) IFA Clift Assay
2) IFA Hep 2 (screening test, meaning you have to do more testing)
3) RIA Farr Method
What is Hep 2 used as?
Broad screening device for connective tissue disorders. (more appropriate for developing countries)