Cytometry

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Author:
LT24
ID:
5073
Filename:
Cytometry
Updated:
2010-01-25 02:43:22
Tags:
Cytometry
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Description:
Reading cytometry
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  1. Applications of cytometry
    • Visualization of samples on a single cell level
    • Can find a rare cell type among a sample comprised mostly of non-relevant events
    • Sorting of distinct populations within a heterogenous sample
    • Widely used in immunology
  2. How does it work?
    • Sample of cells labeled with antibodies conjugated to fluorochromes
    • Cell suspension is forced into a tiny stream of liquid, so that only one cell enters the machine at a time
    • Each cell, and any antibodies that are bound to it, intercept the laser beam and are excited to a higher energy state
    • The energy is released as a photon of light
    • Attached computer interprets the data and presents a visual display
  3. Antibody staining

  4. CD8 Histogram

  5. CD45 Histogram

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