Q2 microbial genetics
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Is there meiosis in bacterial replication?
Are bacteria haploid or diploid?
What is the gene pool that contains necessary enzymes for survival, biosynthesis etc.
Core gene pool
What type of gene pool does a chromosome belong to?
What kind of genetic elements are part of the flexible gene pool?
- Genomic islands
- Genomic islets
Term for large chunk of DNA planted into a bacterial chromosome
3 mechanisms of horizontal gene transfer
Horizontal gene transfer mechanism characterized by transfer of genetic material by viral delivery
Horizontal gene transfer mechanism characterized by transfer of genetic material by plasmid
Horizontal gene transfer mechanism characterized by transfer of free DNA
In a transformation event, how is free DNA taken up into the chromosome?
What is required for general recombination? (2)
- Large region of homology
- Rec genes on chromosome
Term for ability of bacteria to be transformed
What small peptide is constituively expressed and has a role in making a cell available for transformation?
What does a competence factor interact with to bring about cell competence?
Surface receptor (not present at all times) --> signal transduction
What does a recA protein do?
Helps free DNA find its way to bacterial chromosome for recombination
How does H. influenzae import free DNA into cell for transformation?
- Via a tranformasome
- No competence factor needed
Term for when cells that are competent first produce proteins that kill other cells of the same species --> lysis of cells and spillage of DNA
Term for the regulation of gene expression in response to fluctuations in cell-population density
Bacteriophage that can infect E. coli
2 types of bacteriophage "lifestyles"
Term for new gene product that allows release of bacteriophage from a cells into the environment
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