The flashcards below were created by user
Anonymous
on FreezingBlue Flashcards.
-
coccus shape gram -
Neisseria sp.
-
coccus shape gram +
do a catalase test
-
catalase -
preform Bile esculin hydrolysis
-
-
MSA growth
1. mannitol fermentation
+
_
- +. staphylococcus aureus
- -. staphylococcus epidermidis
-
Bile esculin hydrolysis
- +. Enterococcus faecalis
- -. Hemolysis
-
Hemolysis
Alpha hemolysis= Streptococcus pneumoniaBeta hemolysis= Streptococcus pyogenes
-
-
Gram - Rod
Glucose and oxidase
-
Glucose + Oxidase -
Lactose test
-
-
Urea
- +. Proteus
- -. H2S=+ Salmonella typhimurium
- - Shigella flexneri
-
-
Indole+,-
- + E. coli
- - H2S + Citrobacter freundil
- - Citrobacter diversus
-
Indole-
- Motility
- +Enterobacter aerogenes
- -Klebsiella pneumonia
-
-
Acidic stain
Basic stain
Spore stain
Acid Fast stain
Capsule stain
- .carries a negative charge
- .carries a positive
- .useful for staining Clostridium
- .useful for staining Mycobacterium
- .requires a basic stain and an acidic stain
-
The order gram stain reagents are applied is:
crystal violet, iodine, alcohol, safranin
-
Complete this sentence: Negative staining procedures will:
stain the background, not the cell.
-
After being gram stained, gram positive bacteria are:
Blue
-
Gram negative bacteria have:
a thin layer of peptidoglycan in their cell wall
-
The following is a differential stain:
Gram stain
-
The organism on the upper half of this MSA plate (surrounded by a yellow halo) is what organism?
Staphylococcus aureus
-
The medium best suited for differentiation of Streptococci is
blood agar
-
An isolate is a gram-positive coccus, catalase-negative, and optochin-resistant. It is most likely:
viridans streptococci
-
Some streptococcal hemolysins can completely lyse red blood cells resulting in a clear zone around colonies on blood agar. This reaction is called:
beta-hemolysis
-
Staphylococcus epidermidis is able to grow on MSA. It tolerates the high salt but is unable to ferment the sugar in the medium. The sugar is:
mannitol
-
The genus Neisseria is characterized by
gram-negative diplococci with flattened adjacent sides
-
The organism that causes strep throat is
Streptococcus pyogenes
-
The most widely used method to evaluate the effectiveness of antibiotics against specific bacteria is
Kirby-Bauer method
-
Which of the following genera include pathogens of the intestinal tract?
Shigella
-
What color is an uninoculated citrate tube?
Green
-
Which medium is best for differentiating Streptococci?
Blood agar
-
Which medium is best for differentiating Staphylococci?
Mannitol Salt agar
-
Which medium is best for differentiating enterics?
Kligler Iron agar
-
Which medium would you choose to test for indol production?
pink or red
-
A KIA tube is inoculated and grown for 24 hours. It has a black butt, red slant, and no cracks or fissures. This is indicative of:
Glucose fermentation only
-
Which of the following KIA reactions will you most likely observe after growing E. Coli for 24 hours in a KIA tube?
A/A
-
The results of an IMViC series of tests is ++--. What color is at the surface of the SIM tube?
Red
-
The results of an IMViC series of test is --++. What color is the methyl red tube?
yellow
-
A sample of dirt is boiled for in water 10 minutes to kill all vegetative cells. After cooling, you inoculate a TSA plate with a small amount of the dirt. Several colonies grow overnight. The colonies are most likely of which genus?
-
Media in which some metabolic activity of an organism can be detected by inspection of the growth of the organism on the medium are referred to as:
Differential
-
The carbohydrate in MSA is
mannitol
-
Beta-hemolytic, bacitracin sensitive streptococci are growing well on a culture medium. The most likely organism is:
Staphylococcus pyogenes
-
Chocolate agar is used for growing fastidious bacteria.
True
-
Which of the following genera include pathogens of the intestinal tract?
Shigella
-
An isolate is a gram-positive coccus, catalase-negative, and optochin-susceptible. It is most likely
Staphylococcus pneumoniae
-
The mold hyphae you are viewing with your nouveau French microscope appears 156 times normal size. The ocular lens has a power of 12X. What is the power of the objective lens?
13
|
|