Describe the mechanism of the Bohr affect. What is Bohr his? HOw does this change the pKa, affinity for H+, and acid strength of deoxyHb?
Following conformational change upon deoxygenation, three residues of Hb are in a more (-) environment. These are HIS 146 (Bohr HIS), HIS 122, and alpha amino group of the alpha chain. This increase the pKa of Hb, increases the affinity of H+, and makes deoxy Hb and weaker acid.