Introduction to Forensic Toxicology
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What is toxicology?
It encompasses the measurement of alcohol, drugs, and other toxic substance in biological specimens and interpretation of such results in a medico legal context.
Name some examples of specimens sent to a toxicology lab.
- vitreous humor
- gastric contents
- tissues (eg liver)
- decomposition fluid
- all hospital samples
Volatiles, EMIT, DOA, GC/MS, and CO-oximetry/Conway diffusion are all examples of ______ ______.
Volatile analysis by HS-GC detects what four substances?
isopropanol, ethanol, methanol, acetone
HS-GC is mainly performed on ______ and ______.
blood and vitreous
T/F: All positive samples with HS-GC are tested twice to ensure results correlate.
True!! But only the lower value is reported.
What does EMIT stand for and what types of samples would you test with it?
Enzyme Multiplied Immunoassay Technique
Urine samples only
What does ELISA stand for and what types of samples do you test with it?
Enzyme Linked Immunoassay Assay
blood, vitreous, serum/plasma, tissue homogenates
ATOX is what kind of drug extraction?
BTOX is what type of drug extraction?
What does CO-oximetry screen?
blood for carbon monoxide
The presence of carboxyhemoglobin is confirmed by which test?
What reagent is in the center well in a conway microdiffusion plate?
What two things are in the conway microdiffusion outer ring?
blood mixed with acetic acid
Vitreous chemistry tests detect the presence of what components?
sodium, chlorine, glucose, urea nitrogen, potassium
T/F: Vitreous chemistry can indicate signs of dehydration/
Most toxicology assays achieve confirmation without ______.
What would you like to do?
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