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kickenbugg
on FreezingBlue Flashcards.
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DNA Ligase
enzyme that catalyzes the joining of DNA fragments
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recombinant DNA
a DNA molecule made in a lab from 2 or more different sources
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sticky ends
the ends of the cut sites where complimentary sticky ends can attatch
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clone
to create an identical copy
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transformation
a cloning process where DNA is cloned by inserting it into host cells
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transfection
transformation of animal cells specifically
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transgenic
a host cell/organism that contains recombinant DNA
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selectable measure
- allow growth of transgenic genes
- EX- genes resistant to antibiotics
- included in recumbant DNA sequence
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replicon
replication unit
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vector
- a carrier DNA squence
- can enter host cell via this
- has its own origin of replication
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reporter gene
any gene whose expression is easily observed
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genomic library
a collection of DNA fragments that together comprise the genome of an organism
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Complimentary DNA (cDNA)
- makes up a much smaller DNA library
- made from transcribed mRNA
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cDNA library
a collection of cDNAs
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knockout
a manipulation that inactivates the gene to keep it from transcribing and being translated
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homologous recombination
a technique to produce a knockout mutant
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stem cell
an unspecialized cell that divides and differenciates into specialized cells
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RNA interface (RNAi)
a rare natural mechanism for inhibiting mRNA translation
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DNA microarray
- technique that allows for hybridization
- provides large arrays of sequences for hybridization expirements
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biotechnology
the use of cell/organisms to produce useful materials
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expression vectors
vectors that have all the characteristics of typical vectors along with other sequences needed for transgene
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pharming
the production of medicine in farm animals/plants
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bioremediation
the use of other organisms to remove contaminants from the environment
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