Micro Lab

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Author:
Anonymous
ID:
54166
Filename:
Micro Lab
Updated:
2010-12-07 19:48:44
Tags:
Micro Utah
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Description:
Lab Final
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  1. Simple stains Basic
    Methylene Blue, Safranin, Crystal Violet, or Malachite Green
  2. Simple Stains Acidic
    Eosin, Congo Red or Nigrosine
  3. Gram Stain
    • Differential Stain
    • Crystal Violet, Iodine, Alcohol, Safranin
  4. Capsule Stain
    Congo Red, India Ink, Crystal Violet
  5. Endospore Stain
    • Bacillus, Clostridium
    • Malachite Green, Steam, Safranin
  6. Acid-Fast Stain
    • Mycobacterium (TB, Leprae)
    • Red = +
    • Blue = -
  7. Motility
    • Use:
    • Motility tube = Red stab
    • SIM tube - cloudiness around stab
  8. H2S Production
    • SIM tube = Black around stab
    • KIA = Black precipitate
  9. Oxygen Requirements
    • Thioglcycolate Tube
    • Aerobes grow at the top
    • Microaerophiles in middle
    • Facultative Anaerobes throughout
    • Anaerobes below the Oxidized layer
  10. UV Light
    Agar plate and look for growth after exposer
  11. Kirby-Bauer Method
    • Muellar Hinton Agar plate
    • + large zone of inhibition
    • - sm or no zone of inhibition
  12. Bile Esculin Hydrolysis
    • For group D strep (faecalis)
    • Black color around growth= +
    • - = no growth
  13. Phenol Red Carb Fermentation
    • Glucose, Lactose, Mannitol
    • = yellow and bubble in tube for gas production
  14. Methyl Red
    • Enterics
    • MR-VP tube
    • add methyl red dye and turns red
  15. Voges-Proskauer
    • Enterics
    • Add Reagent A and B
    • + = pink or red color
  16. Nitrate reduction
    • Enterics
    • Nitrate Broth
    • Add test reagents A and B
    • + = red through out tube
  17. Oxidase Production
    • TSA plate
    • add oxidase reagent
    • + = blue or purple in 30sec
  18. Staphylococcus ID
    • S. Aureus = MSA yellow clear Blood Agar
    • S. Epidermidis =MSA pink
  19. Streak-Stab Agar Plate
    Strept ID
    • S. pyogenes = inhibition around A disk
    • S. pneumonia = inhibition around P disk
    • S. agalactiae = inhibition around CAMP
  20. Starch Hydrolysis
    • Bacillus
    • Starch agar plate
    • clear growth around it
  21. Indole
    • SIM, TSB, Tyrpotone Broth
    • add Kovac's reagents
    • + = red layer at top
  22. Urea Hydrolysis
    • Urea Broth
    • Phenol red in broth turns red or pink
  23. KIA
    • All are rods
    • Enterbacteriaceae
    • Yellow = Acid
    • Red = Base
  24. IMViC
    • All are rods
    • Enterobacteriaceae
  25. Bacillus
    • GRM + Rod
    • TSA Catalase + Starch agar +
  26. Citrobacter
    • GRM - Rod
    • MAC/EMB, KIA, Urea
  27. Clostridium
    • GRM + Rod
    • TSA in anaerobic Jar
    • Catalase -
  28. Endospores
    • Clostridium and bacillus
    • Blood agar, Starch, TSA
  29. Neisseria
    • GRM - diplococcus
    • chocolate agar
  30. Pseudormonas
    • GRM - rod
    • KIA,MAC&EMB, TSA
  31. Salmonella
    • GRM - Rod
    • KIA, MAC & EMB
  32. Staph aureus
    • GRM + Coccus
    • Blood Agar beta
    • MSA= yellow
  33. Staph epidermidis
    • GRM + coccus
    • Blood agar no hemolysis
    • MSA pink or white
  34. Strep pyogenes
    • GRM + Coccus chain
    • Blood Agar beta hemolysis
    • A disk sensitive
  35. E. coli
    • GRM - Rod single
    • EMB green
    • MAC pink
  36. Enterbacter aerogenes
    • GRM - rod single
    • EMB dark pigment
    • MAC pink
  37. Klebsiella pneumonia
    • GRM - Rod single
    • EMB(KIA IMViC) Dark pig
    • MAC pink
  38. Shigella
    • GRM - Rod single
    • EMB (KIA IMViC) colorless
    • MAC colorless

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