The flashcards below were created by user
on FreezingBlue Flashcards.
What functions do enzymes play in the body?
- They are catalysts.
- They speed up the rate of chemical reactions in our body.
Enzymes are which type of substance?
What things can denature enzymes?
- Strong Acid or base
- Vigorous Shaking
- UV radiation
What is a substrate?
The material acted upon by the enzyme.
How are enzymes named?
- Name of substrate
- Tells what kind of reaction and ends in -ase.
- ex. glucose oxidase
Where are enzymes normally found?
In Intact cells
Alkaline phosphatase (ALP or ALKP) an diestive enzyme is active in what pH?
- Work best at pH 10,
- High pH optimum pH 10
Alkaline phosphatase increased in what kind of cancer?
- Bone disease
- Liver disease
SGOT / AST
Increased in what diseases?
SGPT / ALT a transferase enzyme is increased in only what disease?
CPK aka CK are found in which tissues?
What are the 3 isoenzymes and which tissue is each from?
- CK1 - 2 BB chains from Brain and Lung
- CK2 - MB chain from Heart
- CK3 - MM chain from Muscle
What is important about LDH?
- Present in almost all tissues.
- Remains elevated longest in MI.
How are isoenzymes separated?
5 isoenzymes separated by Electrophoresis
Why are hemolyzed specimens not acceptable?
High concentration in RBC
Which 2 enzymes are ordered together to evaluate pancreatic functions?
Amylase and Lipase
Which test are in a cardic panel?
- CPK or CK
- Troponin I adnormal protein specific to heart
- Troponin T adnormal protein specific to the heart and muscle, best predictor to ACS and MI.
Which test are in a liver profile?
- GGT, GGTP or GT
Which is the most sensitive of the liver enzymes?
GGTP (gin and tonic)