Micro lec 2
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Name the different bacterial morphology.
bacterial cytoplasm have an overall...
- negative charge, which will attract anything with a positive charged stain
- examples: crystal violet, methylene blue
What stain uses a single agent and is a basic stain with
This stain divides bacteria into groups based on their
staining properties. Give examples
- Examples: gram stain,
- acid-fasting stain
stain divides bacteria into two groups, examples.
Gram stain; gram positive and gram negative
color would stain gram positive and gram negative?
Gram positive – purple
Gram negative – pink
Give the kinds of substances used for each step for
Step1. Crystal violent
Step3. Alcohol wash
- Step4. Safranin
What’s the key point in gram staining?
does a bacteria’s cell wall consist of?
Contains polysaccharide/protein (which is the peptidoglycan)
What gives the cell wall its’ rigidity?
peptidoglycan is found exclusively in..?
What happens to the bacteria when the peptidoglycan is destroyed?
it weakens or kills the bacteria since they become targets for antibiotics
Penicillin is effective toward?
toward gram positive bacteria
in gram positive bacteria, the cell wall...
peptidoglycan is on the outside of the membrane
in a gram negative bacteria, the cell wall..
outer membrane covers peptidoglycan; membrane double barrier is called periplarm
the coating outside the cell wall, slimy gooey layer, an extra layer of protection
what's the function of glycocalyx
for protection, motility, and attachment
Name surface features of the bacteria.
pili and flagella
Whats the function of flagella?
whats the function of pili
attachment, also as information transfering (DNA transfer, conjugation)
List the cytoplasm structures found in a bacteria.
ribosomes, nucleoid, inclusions, plasmids, and sometimes endospores
Endospores. what are they?
only found in some bacteria; considered highly resistant dormant form of the cell; and only forms in time of stress in the environment
its a site for protein synthesis
single closed loop of DNA
smaller loops of DNA nonessential cell functions
growth is consider..
when there is an increase of number of cells or cell size
culture can be either...
liquid form (broth) or solid (agar)
dont know how, but know it works; unknown concentrations/compounds
list the different types of media
General purpose; enriched media, selective media
list general purpose media
- TSA Tryptic Soy Agar
- TSB - broth
Enriched media is?
- general purpose media with something extra
- ex. blood agar
selective media is...
favors growth of some over others
who coined the term "filterable virus"
Dmitri Iwanowski when he was interest in tobaccoo mosaic disease
who brought in electron microscope?
What would you like to do?
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