Automated Cell Counts
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Who invented 1st automated cell counter?
Types of Hematology Analyzers
- Particle Cell Counter (Electrical Impedance)
- Optical Cell Counter (Optical Light Scattering Scanner)
- Radio Frequency (RF) Resistance
- Cells do not conduct electricity.
- Current is broken every time a cells is passed through electrodes.
- High frequency electromagnetic current flows between 2 electrodes.
- Cell wall acts as a conductor to gauge size based on pulse.
- Similar to Impedance but higher frequency.
- Directs Diluted Cells through quartz flow cell in single file.
- Laser light directed at the flow cell is scattered in all directions.
- Photo-detectors at different angles detect scattered light and create a countable pulse.
Low angle light scatter.
2-3o = cell size and volume
High Angle Light Scatter
5-15o = internal complexity
Right Angle Light Scatter
- 90o Side Scatter
- Relates to internal components.
Volume is measured with... (VCS)
Using low frequency current.
Conductivity measured with....(VCF)
High frequency current measures changes in conduction.
Light Scatter (VCF)
Laser light used to differentiate WBCs based on surface shape and reflectability.
Impedence/VCS Technology Analyzer
Impedance/Hydrodynamic Focusing Analyzer
Optical Scanner/Impedance Analyzer
Abbott Cell Dyn
What is measured in a Automated CBC
- Platelet Count
- RBC Indices
- Platelet Indices
- 5 Part Diff- Segs, eos, basos, lymphs, mosos
- Reticulocyte count with newest types
Sweep Flow Technology eleminates what?
Double cell Counting
RBC Threshold Limit
Platelet Threshold Limit
WBC Threshold Limit
Histogram Left to Right
Lymphs ->Monos -> Grans
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