clin path lab hematology

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clin path lab hematology
2011-02-23 13:46:35
clin path lab

clin path lab
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  1. avg of slides + 10% x 100 = mm3, using 10x
    WBC count of hemacytomer formula
  2. avg of 10 fields x 2000 = mm3, using 40x
    WBC estimation formula
  3. avg of 10 fields x 20,000 = ul, using 100x
    platelet estimation formula
  4. smallest #- largest # = /100x
    platelet range formula
  5. arm
    supports the eyepiece and tube
  6. base
    firm support that bears the weight of the microscope, also used to support the microscope while carrying
  7. coarse focus
    moves the stage up or down approximately to the correct distance
  8. fine focus
    permits exact focusing by moving the stage up or down
  9. condenser
    condenses and focuses the light coming from the source.
  10. binocular
    having two eyepieces. allow for interpupillary adjustment.
  11. iris diaphragm
    device between the light source and the stage that is used to vary the amount of light that passes through the subject
  12. illuminator
    directs light upward though the iris diaphragm and the condenser
  13. eyepiece
    the lens closest to the eye when looking through a microscope
  14. eyepiece tube
    holds the ocular and objective lenses at the proper working distance from each other
  15. nosepiece
    permits interchange of low and high power lenses
  16. mechanical stage
    a device, which is mounted on the microscope stage, and by means of two dials allows precise movement and positioning of the slide.
  17. oil immersion
    lens that requires a drop of special oil on the subject for use.
  18. objective lens
    closest to the specimen
  19. ocular lens
    closest to the eye, magnifies 10x
  20. scanning objective
    magnifies 4x, usually with a red ring
  21. low power objective
    maginifies 10x, used to determine cell distribution
  22. high power or high dry objective
    magnifies 40x, used to find a good counting area
  23. oil immersion objective
    100x, used to get your actual count...if you can't focus on this your slide might be upside down!
  24. objective lens x ocular lens =
    number of times magnified
  25. proper allignment of the illuminating light for microscopy
    koehler illumination
  26. wright's stain
    polychrome stain
  27. Methylene blue derivatives
    blue stain
  28. eosin stain
    orange stain
  29. serpentince counting pattern
    counting pattern of a smear
  30. size, nucleus, cytoplasm
    3 factors of WBC identification
  31. adequate
    clumping in feathered edge, if no platelets found in counting area
  32. 11-29/100x, 11-26/100x
    cat and dog platelet range
  33. normal
    clear to straw color
  34. reddish
  35. yellow
  36. milky white
  37. hypoproteinemia - below 6g/dl
    malnutrition, liver disease, renal disease, pregnancy, lactation, normal in young animals (6months or less)
  38. hyperproteinemia - above 7.5 g/dl
    shock, neoplasms (tumors), infections, acute pancreatitis, (FIP) Feline Infectious Peritonitis, lipemia gives false readings
  39. acanthocytes
    erythrocytes with abnormal blunt projections
  40. anisocytosis
    variation in size of erythrocytes
  41. atypical
    not normal
  42. autoaggluination
    spontaneous clumping or agglutination of erythrocytes in drawn blood
  43. basophillic
    capability of staining various intensities of blue with Wright's stain
  44. crenation
    the margins of erythrocytes appearing as sharp points
  45. dacrocytes
    tear drop shape, associated with bone marrow disorders
  46. echinocytes
    burr cells are spiculated erythrocytes with 10-30 equally spaced projections. very similar to crenation
  47. erythremic myelosis
    abnormal proliferation of erythrocytic cells in peripheral blood and bone marrow
  48. erythrocyte refractile bodies
    (ER bodies) same as Heinz bodies
  49. erythrocytes
    mature red blood cells
  50. erythroid cells
    cells of the erythrocytic series
  51. heinz bodies (ER bodies)
    refractile inclusions of denatured hemoglobin often noted in cat erythrocytes. These inclusions may cause bulging of the erythrocytes. With new methylene blue stain the bodies are blue-black.
  52. howell-jolly bodies
    retained round nucleur fragments within the erythrocytes
  53. hypocromasia
    decreased hemoglobin in erythrocytes causing lighter color when stained
  54. leptocytes
    thin erythrocytes with increased membrane or decreased volume. The most common form in the Target cell or folded cell
  55. macrocytes
    red blood cells that are larger than normal, with an increased MCV. Macrocytes are usually young, polychromatophilic erythrocytes (Reticulocytes)
  56. metarubricytes
    Immature red blood cells in a stage of development where the nucleus appears as a dark blue homogenous mass without any distinct chromatin structure.
  57. microcytes
    red blood cells that have a diameter less than that of a normal red blood cell. A decreased MCV. Microcytes have been seen with iron deficiency
  58. poikilocytosis
    the presence of irregularly shaped red blood cells in periphearal blood
  59. 37-45%
    canine PCV
  60. 24-45%
    Feline PVC
  61. 12-18 g/dl
    canine hemoglobin
  62. 8-15 g/dl
    feline hemoglobin
  63. 5.5-8.5 x 106
    canine RBC count
  64. 5-10 x 106
    feline RBC
  65. 6.0-7.5 g/dl
    canine and feline TP
  66. 6-17,000 mm3
    canine WBC
  67. 5.5-19,000 mm3
    feline WBC
  68. 60-75%
    canine segs
  69. 35-75%
    feline segs
  70. 0-3%
    canine and feline bands
  71. 12-30%
    canine lymphs
  72. 20-55%
    feline lymphs
  73. 3-10%
    canine monos
  74. 1-4%
    feline monos
  75. 2-10%
    canine EOS
  76. 2-12%
    feline EOS
  77. 0%
    canine and feline basos
  78. 200,000-500,000/ul
    canine platelets
  79. 300,000-700,000/ul
    feline platelets
  80. The tendency of red blood cells to stain basophilic, young erythrocitic cells. Synonym is basophilia.
  81. developing cells in the stage from rubriblasts to rubricytes.
  82. there is evidence of increased red blood cell production in the blood and bone marrow (polychromasia, reticulocytosis)
    regenerative anemia
  83. young, large erythrocytes that contain a blue reticulum when stained with new methylene blue stain
  84. red blood cells affixed in groups resembling a stack of coins
    rouleau (rouleaux)
  85. immature cells in stage of development from stem cells to prorubricytes
  86. immature blood cells in state of development to metarubricytes
  87. rubriblasts, prorubricytes, rubricytes, metarubricytes
    order of blood cell development
  88. irregularly shaped fragments of erythrocytes. These are cells that have been sheared into pieces, often taking a helmet shape.
    schistocytes, helmet cells
  89. dense spherical canine erythrocytes that appear to be smaller than normal erythrocytes
  90. erythrocytes with oval-shaped areas of central pallor and are observed in hereditary stomatocytosis of Alaskan Malamutes.
  91. star shaped
  92. irregulary shaped erythrocytes with the appearance of a target
    target cells