o In bacteria, the small subunit can bind these two in either order; it binds the mRNA at a specific RNA sequence, just __of the start codon, __.
o In eukaryotes, the small subunit, with the __already bound, binds to the 5’ cap of the mRNA and then moves, or scans, __along the mRNA until it reaches the start codon, and the initiator tRNA __to it.
o In either case, the start codon signals the start of __; this is important because it establishes the __for the mRNA.
- initiator tRNA
- hydrogen bonds
- codon reading frame