Bio6L

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Author:
lukemlj
ID:
66234
Filename:
Bio6L
Updated:
2011-02-14 02:36:18
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Bio6L Lab Safety
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Bio6L Lab Safety
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  1. The four routes of possible CHEMICAL exposure are:
    • 1) Inhalation of powder, vapor or gas
    • 2) Ingestion by mouth
    • 3) Puncturing / injection thru skin
    • 4) Absorbing thru skin
  2. Thinner skin results in _____ rates of absorption.
    higher
  3. Six Warning Signs of Chemical Exposure
    • Breathing difficulties – faster, deeper, soreness or a lump in the throat feeling.
    • Dizziness, drowsiness, disorientation or difficulty concentrating.
    • Burning sensation, redness or soreness in the eyes or on the skin.
    • Weakness, fatigue or lack of energy.
    • Chills or upset stomach.
    • Odors or a strange (metallic) taste in your mouth.
  4. What does MSDS stand for?
    Material Safety Data Sheet
  5. What does the MSDS do?
    THE MSDS TELLS YOU EVERYTHING YOU NEED TO KNOW IN ORDER TO WORK SAFELY WITH A CHEMICAL.
  6. Name 4 chemical categories.
    • Ignitability
    • Reactivity
    • Toxicity
    • Corrosivity
  7. What are 2 categories of Ignitablity?
    • Flammable
    • Combustible
  8. What are 3 categories of Reacitivty?
    • Explosive
    • Oxidative
    • Water reactive
  9. What are 3 categories of Toxicity?
    • Acute (poisons)
    • Chronic/Cumulative
    • Non-toxic but with health effects in concentration
  10. What are 2 categories of corrosivity?
    • Acids & Bases
    • Solvents
  11. What makes up the Fire Triangle?
    • Fuel
    • Oxygen
    • Heat/Energy
  12. Name 4 Chemical Compatibility Rules
    • Separate acidic chemicals from basic chemicals according to pH
    • Separate oxidizers from organic chemicals & inorganic reducing agents
    • Separate water reactive chemicals from aqueous solutions
    • Separate chemicals with multiple hazards from all incompatible chemicals
  13. Name 5 general chemical reactions.
    • Corrosives + Flammables => Explosion / Fire
    • Corrosives + Poisons ===> Poison Gas
    • Flammables + Oxidizers ==> Explosion / Fire
    • Strong acids + Strong Bases ===> Heat / Salts
    • Water reactives + Water ===> Toxic or Flammable Gas
  14. Hazardous wastes are ...
    used, spent or expired chemicals which fall under the chemical categories of being flammable, reactive, toxic or corrosive.
  15. Usually, __________ causes chemical accidents. Estimated ____% of all lab accidents involving chemicals is due to mixing & mishandling of waste. Why?
    • human error
    • 85-90
    • Chemical compatibility and/or segregation is not adhered to.
  16. Name two important pieces of information on a hazardous waste label.
    • Accumulation start date
    • SPECIFIC CHEMICAL NAME(s), CONCENTRATION OR AMOUNT, APPROXIMATE PERCENT
  17. Name a few appropriate acronyms for a chemical label.
    SDS, EDTA, Tris, PBS
  18. Define a pathogen
    An agent that is capable of causing disease
  19. Draw the chain of infection
    • 1) Carrier/reservoir (quarantine)
    • 2) Portal of exit (mask)
    • 3) Mode of transmission (wash hands)
    • 4) Susceptible host (vaccination)
    • 5) Portal of entry (mask)
  20. Indirect portal of entry is at least ___ feet from portal of exit.
    6
  21. What is a fomite?
    Inanimate object capable of harboring disease.
  22. What are 5 biological modes of transmission?
    • Ingestion
    • Inhalation
    • Contact
    • Trauma
    • Vector borne
  23. What are the 5 F's?
    • Fingers
    • Food
    • Flies
    • Feces
    • Fomites
  24. What are three types of arthropod-borne infections and zoonoses?
    • Vector (biting arthropod), e.g. malaria
    • Vertebrate reservoir, e.g. rabies
    • Vector (vertebrate), e.g. Black Plague
  25. Name 5 "Other Hazards"
    • Electrical
    • Liquid nitrogen
    • Radioactive material
    • UV radiation
    • Centrifuges
  26. Gel Electrophoresis unit uses how many amps?
    23
  27. What basic PPEs are required for handling liquid nitrogen?
    • Lab coat
    • Face shield
    • Cryogenic gloves
  28. What is a big safety concern regarding centrifuges?
    Balance
  29. What tool for looking at gels uses UV radiation?
    Transilluminators

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