Card Set Information
Bio6L Lab Safety
Bio6L Lab Safety
The four routes of possible CHEMICAL exposure are:
1) Inhalation of powder, vapor or gas
2) Ingestion by mouth
3) Puncturing / injection thru skin
4) Absorbing thru skin
Thinner skin results in _____ rates of absorption.
Six Warning Signs of Chemical Exposure
Breathing difficulties – faster, deeper, soreness or a lump in the throat feeling.
Dizziness, drowsiness, disorientation or difficulty concentrating.
Burning sensation, redness or soreness in the eyes or on the skin.
Weakness, fatigue or lack of energy.
Chills or upset stomach.
Odors or a strange (metallic) taste in your mouth.
What does MSDS stand for?
Material Safety Data Sheet
What does the MSDS do?
THE MSDS TELLS YOU EVERYTHING YOU NEED TO KNOW IN ORDER TO WORK SAFELY WITH A CHEMICAL.
Name 4 chemical categories.
What are 2 categories of Ignitablity?
What are 3 categories of Reacitivty?
What are 3 categories of Toxicity?
Non-toxic but with health effects in concentration
What are 2 categories of corrosivity?
Acids & Bases
What makes up the Fire Triangle?
Name 4 Chemical Compatibility Rules
Separate acidic chemicals from basic chemicals according to pH
Separate oxidizers from organic chemicals & inorganic reducing agents
Separate water reactive chemicals from aqueous solutions
Separate chemicals with multiple hazards from all incompatible chemicals
Name 5 general chemical reactions.
Corrosives + Flammables => Explosion / Fire
Corrosives + Poisons ===> Poison Gas
Flammables + Oxidizers ==> Explosion / Fire
Strong acids + Strong Bases ===> Heat / Salts
Water reactives + Water ===> Toxic or Flammable Gas
Hazardous wastes are ...
used, spent or expired chemicals which fall under the chemical categories of being flammable, reactive, toxic or corrosive.
Usually, __________ causes chemical accidents. Estimated ____% of all lab accidents involving chemicals is due to mixing & mishandling of waste. Why?
Chemical compatibility and/or segregation is not adhered to.
Name two important pieces of information on a hazardous waste label.
Accumulation start date
SPECIFIC CHEMICAL NAME(s), CONCENTRATION OR AMOUNT, APPROXIMATE PERCENT
Name a few appropriate acronyms for a chemical label.
SDS, EDTA, Tris, PBS
Define a pathogen
An agent that is capable of causing disease
Draw the chain of infection
1) Carrier/reservoir (quarantine)
2) Portal of exit (mask)
3) Mode of transmission (wash hands)
4) Susceptible host (vaccination)
5) Portal of entry (mask)
Indirect portal of entry is at least ___ feet from portal of exit.
What is a fomite?
Inanimate object capable of harboring disease.
What are 5 biological modes of transmission?
What are the 5 F's?
What are three types of arthropod-borne infections and zoonoses?
Vector (biting arthropod), e.g. malaria
Vertebrate reservoir, e.g. rabies
Vector (vertebrate), e.g. Black Plague
Name 5 "Other Hazards"
Gel Electrophoresis unit uses how many amps?
What basic PPEs are required for handling liquid nitrogen?
What is a big safety concern regarding centrifuges?
What tool for looking at gels uses UV radiation?