Enzyme kinetics/inhibitors

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Author:
bryan69604
ID:
66892
Filename:
Enzyme kinetics/inhibitors
Updated:
2011-02-16 21:04:47
Tags:
enzyme kinetics michaelis menten reversible inhibition
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Description:
Michaelis-menten enzymes, effect of reversible inhibitors on Km/Vmax
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  1. Michaelis-Menten eqn
    • V0 = Vmax [S]
    • Km + [S]
  2. Assumptions of MM eqn
    • -Rate is V0 --> ignore k4
    • -Single S & ES
    • -Constant [ES]
    • -[S]>>>[E]
  3. Linear form of MM eqn
    1/V0 = (Km/Vmax)x(1/[S]) + 1/Vmax
  4. Ki
    • [E][I]
    • [EI]
  5. Competitive I
    • same Vmax
    • higher Km
  6. Non-competitive I
    • lower Vmax
    • same Km
    • (binds E & ES equally)
  7. Mixed I - preference for E
    • lower Vmax
    • raise Km
    • (lowers k1 more than k2+k3)
  8. Mixed I - preference for ES
    • lower Vmax
    • lower Km
    • (lowers k2+k3 more than k1)
  9. Uncompetitive I
    • lower Vmax
    • lower Km
    • (to same extent --> all lines on lineweaver-burke plot same slope)

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