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What is a mutation?
A mistake in the cells genetic material
How is a wiled type DNA altered?
What causes Mutations?
What are the two ways to combat mutation?
- Reversion- healing (rare)
- DNA repair mechanism- restoring damaged DNA (mismatch repair, excision repair)
Through which cells are mutations inherited, and which cells are not?
- Germ line- inherited
- Somatic- not
What two things can human disorders be?
- Autosomal chromosome disorder (Homozygous recessive disorder)
- Sex-linked disorders
What is gene therapy?
replacing a defected gene with a good copy of the gene
What are the methods of delivery for gene therapy?
- Adenovirus (DNA)
- Retrovirus (RNA)
- Particle bombardment
What are the two ways of doing molecular fingerprinting?
PCR and RFLP
What is the procedure for RFLP?
- RE digest
- Southern blotting
- Visualizing by autoradiograph
How does PCR work for DNA fingerprinting?
Amplify DNA fragment showing hyper variable region and discriminate the probe