At the replication fork, strand have to unwound, what enzymes assist?
At the rep fork, the DNA double helix is unwound by ther enzyme helicase, allowing the two DNA strands to be separate, which now become template strands for the synthesis of new DNA. The two strands of DNA are unstable, but single strand binding proteins line up along the unpaired DNA strands, allowing them to remain apart for the synthesis of the daughter strands. Another enzyme called topoisomerase relieves strain caused by the untwisting of the double helix, which causes tighter strain caused by the untwisting of the double helix, which causes tighter twisting and strain ahead of the rep fork. Topoisomerase accomplishes this by breaking, swiveling, and rejoining the DNA strands.