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An unbranched chain of ribonucleotides joined by 3’-5’ phosphodiester bonds
What subunits is RNAP made up of?
This part of RNAP identifies the promoter region
This subunit of RNAP sticks to the DNA
Beta prime subunit
What does Rifampicin do?
- Inhibits Beta subunit
- stops bond formation
What does actinomycin do?
- Sticks to DNA
- Stops it from unwinding
RNA binds to DNA in this stage of transcription
Sigma goes away and RNA is made in this stage of transcription
The hairpin loop is made in this stage of transcription
Transcription and translation happen at the same time in these guys
How many types of RNAP are in prokaryotes?
These types of RNAP are in eukaryotes
This type of RNAP makes the primary transcript
Adding a piece of 7-methyl-guanosine to the 5’ end of mRNA is called this
This enzyme does the 5' capping
What does the 5' cap do?
- starts translation
Adding a couple hundred Adenines to the 3’ end of mRNA is called this
The sequence of mRNA signals the whole “A-tailing”
- Polyadenylation sequence
What does the Poly A tail do?
- Helps it get out of nucleus
Removing the introns is called this
This machine does the actual splicing
What is a splicosome made up of?
snRNA and proteins
These two things help make diverse amount of proteins from limited genes
- Alternative splicing
- RNA editing
shoving a STOP codon into the mRNA by surprise is called this
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