translesion dna synthesis
Once the lesion is generated in the DNA, the replication machine stall, now either the lesion is repaired or bypass by specialized polymerases. Proliferating cell nuclear antigen (PCNA) plays instrumental role in translesion synthesis (TLS) by providing the platform to TLS polymerases for efficient bypass of the lesions. In normal scenario DNA is replicated by polymerase delta bound to PCNA and other accessory proteins. At the site of lesion, replication is stalled and PCNA is ubiquitinated by RAD6/RAD18 proteins dependant manner and polymerase delta is replaced by pol h.