Cell Indices

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Author:
moses1424
ID:
71593
Filename:
Cell Indices
Updated:
2011-03-08 12:59:02
Tags:
hematology
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hematology
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  1. MCV
    Mean Cell volume.
  2. MCH
    Mean Cell hemoglobin.
  3. MCHC
    • Mean Cell Hemoglobin Concentration
    • Average HGB concentration per RBC.
  4. RDW
    Red Cell Distribution Width
  5. MPV
    Mean Platelet Volume.
  6. How is MCV determined?
    Saline Displacment.
  7. How is MCV Calculated?
    HCT/RBC x 100 = MCV
  8. Normal Ranges for Hemoglobin weight?
    27-33 Pg
  9. Less than 27pg hemoglobin?
    Hypochromic
  10. Greater than 33Pg?
    Hyperchromic
  11. MCH calculated how?
    (hgb/rbc)x10 = MCH
  12. How is MCHC determined?
    (HGB/HCT) X 100 = MCHC
  13. Red cell width Distribution normal range?
    11.5-14.5
  14. How is RDW Calculated?
    (1SD/MCV) x 100 = RDW
  15. Mean Cell Platelet Volume normal range?
    • 1-4 microns
    • 6.5-8 Fl
  16. Uncorrected Ret. count calculation?
    #of retic per 1000 rbc's / 10 = uncorrected
  17. Corrected Retic. Count Calculation?
    (Uncorrected Retic. x Patient HCT) / Normal Hct = corrected
  18. RPI calculation?
    RPI = Corrected / days maturity
  19. Days Maturity?
    • HCT/Days
    • 35% / 1.5
    • 30% / 1.75
    • 25% / 2
    • 20% / 2.5
    • 15% / 3
  20. Normal Reticulocyte range?
    .5 -.1.5 %
  21. < 0.5 % reticulocyte
    No bone marrow activity
  22. >2% reticulocyte range
    indicates increased bone marrow activity/
  23. Decreased reticulocyte cause
    • Radiation
    • Immunosuppresive drugs
    • Aplastic Anemia
    • Pernicious anemia
    • Leukemia (late stage)
  24. Increased reticulocyte cause?
    • Blood Loss
    • Hemolytic anemias
    • autoimmune
    • Sickle cells
    • HDN; Hemolytic transfusion reaction
    • Iron Deficiency anemia
    • Thalassemias
  25. Stain for reticulocytes?
    • Supravital Stain
    • New Methylene Blue
    • EDTA sample
  26. RPI
    Reticulocyte Production Index
  27. External solution is hypotonic to cytosol.
    water moves into the cells
  28. External solution is hypertonic to cytosol
    out of cell
  29. External solution is isotonic to cytosol
    no movement
  30. Osmotic fragility?
    Ability of red cells to withstand hemolysis when placed in hypotonic solutions.
  31. Increased fragility causes.
    • Hereditary Spherocytosis.
    • Hemolyti anemias
  32. Hereditary Sperocytosis
    Hemolytic anemia due to RBC membrane defect.
  33. Decreased fragility causes
    • Sicklecell
    • Thalassemias
    • Iron Deficiency
    • Polycythemia Vera
  34. Heriditary eliptocytosis
    • Ovalocytes
    • elliptocytes
    • Normal Fragility
    • Decreased thermal Stability
    • *Become echinocytes and fragments at 47o
  35. Increased CHOL & phosolipids in membrane
    Target Cells
  36. Stomatocytes
    • Rh Null individuals
    • Alchoholism and drug therapy
    • Up to 3% normal
    • Drying artifact

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