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What are the 2 sources of cell culture media?
- In-house - centralized
- Commercial- additional lab prep required
What are the 4 types of media?
- Serum based
- Serum free
- Animal free
- Protein Free
What are the 2 components in a complete culture medium?
What is in a basal medium?
- Nutrients (carbs, amino acid, vitamins)
- Salts and bufferes
What is balanced salt solution (BSS) required to do?
Maintain pH and prevent osmotic imbalance
What are vitamins?
Nutrients required in very small amounts for essential metabolic reactions to help cells growth and multiplication
What is serum?
Yellowish, clear liquid left over after fibrin and cells removed from blood or it is a supernatant of clotted blood
What are the 2 types of continuous culture?
- Cell lines
- Continuous Cell lines
What is transformation?
Spontaneous or induced permanent phenotypic changes resulting from change in DNA and gene expression
What is transfection?
Introduction of DNA into a cell
What is the difference between histotypic culture and oranotypic culture?
- Histo- single cell lineage
- Organ- multiple cell lineage
What are the cell cycle phases?
- G0/G1- pre DNA synthesis
- S - DNA sythesis
- G2 - Period between DNA synthesis and mitosis
- M - mitosis
What is the equation for measuring cell growth?
n= 3.32 (log NH - log N1)
What is cryopresevation?
A process where cells or whole tissue are preserved by cooling to sub-zero
What are major cell culture contaminants?
What are the 3 classifications of bacteria?
- Spiral (spirilli)
- Rod (bacilli)
- Round (cocci)