Cell Culture Final

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Author:
icimmy
ID:
72547
Filename:
Cell Culture Final
Updated:
2011-03-13 11:34:52
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Cell Culture Final
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Cell Culture Final
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  1. What are the 2 sources of cell culture media?
    • In-house - centralized
    • Commercial- additional lab prep required
  2. What are the 4 types of media?
    • Serum based
    • Serum free
    • Animal free
    • Protein Free
  3. What are the 2 components in a complete culture medium?
    • Basal Medium
    • Supplements
  4. What is in a basal medium?
    • Nutrients (carbs, amino acid, vitamins)
    • Salts and bufferes
  5. What is balanced salt solution (BSS) required to do?
    Maintain pH and prevent osmotic imbalance
  6. What are vitamins?
    Nutrients required in very small amounts for essential metabolic reactions to help cells growth and multiplication
  7. What is serum?
    Yellowish, clear liquid left over after fibrin and cells removed from blood or it is a supernatant of clotted blood
  8. What are the 2 types of continuous culture?
    • Cell lines
    • Continuous Cell lines
  9. What is transformation?
    Spontaneous or induced permanent phenotypic changes resulting from change in DNA and gene expression
  10. What is transfection?
    Introduction of DNA into a cell
  11. What is the difference between histotypic culture and oranotypic culture?
    • Histo- single cell lineage
    • Organ- multiple cell lineage
  12. What are the cell cycle phases?
    • G0/G1- pre DNA synthesis
    • S - DNA sythesis
    • G2 - Period between DNA synthesis and mitosis
    • M - mitosis
  13. What is the equation for measuring cell growth?
    n= 3.32 (log NH - log N1)
  14. What is cryopresevation?
    A process where cells or whole tissue are preserved by cooling to sub-zero
  15. What are major cell culture contaminants?
    • Bacteria
    • Fungus
    • Mycoplasma
  16. What are the 3 classifications of bacteria?
    • Spiral (spirilli)
    • Rod (bacilli)
    • Round (cocci)

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