Immuno Final, I

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Immuno Final, I
2011-04-06 12:48:18

Immuno Final, I
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  1. Ab indicates_____, Ag indicates_____
    • Ab= Exposure
    • Ag= Agent
  2. Serology is a measurement of...
    Ag-Ab interactions
  3. Uses of Immunological Tests (10):
    • Detect exposure
    • Quantify Ab or Ag
    • ID infectious agents
    • Monitor exposure
    • Vx titers
    • Monitor proteins, hormones & drug levels
    • Type blood & Tissue
    • Recheck leukemia/lymphoma therapy
    • ID tumor Ag & Auto-Ab
    • ID cells in immune response
    • Survey a population
  4. What is the basis for a variety of immunologic tests?
    Ag-Ab interaction
  5. What affects the Ag-Ab interaction?
    • Forces of attraction
    • Concentration of Ag-Ab
  6. Titration-
    Serum dilutions used to measure the amount of Ab in serum
  7. Purpose of a titer
    Quantify the amount of Ab
  8. What methods can be used to detect Ab for a titration?
    • Precipitation/Agglutination
    • ELISA
  9. Using an ELISA, what would indicate a high titer?
    +wells with color change
  10. Which value indicates a higher Ab concentration, 1:4 or 1:512?
    1:512 = higher Ab concentration
  11. Two general types of Immunodiagnostic Tests:
    • Primary Binding Tests
    • Secondary Binding Tests
  12. Primary Binding Tests are used to....
    Directly measure binding of Ag to Ab in vitro
  13. Secondary binding tests are used to....
    Measure the RESULTS of Ag-Ab interactions in vitro
  14. Samples that can be used for in vitro tests (4):
    • Serum
    • Peritoneal Fluid
    • Pleural Fluid
    • CSF
    • Synovial Fluid
  15. Cells/Tissues to probe with Ab when looking for Ag
    • Whole blood in anticoag (RBC, WBC and Platelets)
    • Tissue Sections on dead or euthanized animals or from biopsy
  16. What Reagents can be used in immunodiagnostic tests(5)?
    • Complement
    • Polyclonal Ab
    • Monoclonal Ab
    • Anti-globulins (Ab to Ab)
    • Anti-serum
  17. Anti-Serum contains...
    Ab against specific Ag
  18. What must ALWAYS be done before running a binding test?
    Run a known positive and negative controls
  19. Two Major Types of Primary Binding Tests:
    • Immunofluorescence Assays
    • Immunoenzyme Assays
  20. Immunofluoroescence Assays include (2):
    • Direct Fluorescent Ab Tests
    • Indirect Fluorescent Ab Tests
  21. What do DFA and IFA detect?
    • Ab
    • (Direct/Indirect Fluorescent Ab)
  22. Immunoenzyme Assays include (3):
    • ELISA
    • Western Blot
    • Immunohistochemistry
  23. Secondary Binding Tests include (4):
    • Precipitation Tests
    • Agglutination Tests
    • Neutralization Tests
    • Complement Fixation Tests
  24. What is the unknown in Direct Flourescent Ab Tests? Indirect FA?
    • Direct= Ag
    • Indirect= Serum
  25. Fluorochrome commonly used to label Ab?
  26. ELISA tests use wells coated with ___
  27. Indication of a Positive ELISA test?
    Color Change
  28. ELISA detects ___
  29. Which ELISA test can be used for a titer?
  30. Which test requires electrophoresis of proteins?
    Western Blot
  31. Western Blot detects ___
    • Protein Ag
    • Ab
  32. Western Blot is probed with:
    • Labeled Ab (protein Ag)
    • P's serum (Ab)
  33. Controls used for an Immunohistochemistry:
    • Irrelevant Ab
    • Normal Tissue Sample
  34. In a Precipitation Test, what does the precipitate consist of?
    Complex of Ab & SOLUBLE Ag