micro.txt

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Anonymous
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76095
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micro.txt
Updated:
2011-03-29 21:49:37
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Microbiology
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test
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  1. Cover the smear with crystal violet for 20 second
    Gram staining
  2. What is the mordent for gram staining
    Gram's iodine
  3. Decolorize the stain with 95 percent ethnal alcohol
    Gram's staining
  4. Cover the stain with safranin for 20 second
    Gram staining
  5. In gram staining bacillus cereus what color do rods stain
    Blue
  6. Is bacillus cereus gram pos or Neg
    Pos
  7. Is escherichia coli gram pos or gram neg
    Neg
  8. Is staphylococcus aureus gram pos or neg
    Pos
  9. In negative staining what stain is used
    Nigrosine
  10. Do you heat fix a negative stain
    No
  11. Place the edge of the clear slide into the nigrosine by backing it
    • into the nigrosine and dragging it foward.
    • Negative staining
  12. Sarcina lutea negatve stain results
    4 cocci, tefrad cellular arrangment
  13. Bacillus cereus negative stain results are long rods
  14. Cover the stain with 1percent aqueous solution of crystal violet
    Capsual staining
  15. Wash with 20percent copper sulfate
    Capsual staining
  16. Do you heat fix a capsular stain
    No
  17. Streptococcus lactis capsular stain results
    Two nucleus in one cell (like an egg)
  18. Enterobacter aerogenes
    Rods longer than ecoli
  19. What is the primary stain used for acid-fast staining
    Carbol fuchsin
  20. What is the mordent for acid fast staining
    Steam heat
  21. What is the decolorizer for acid fast staining
    95percent ethenol alcohol
  22. What is the counter stain for acid fast staining
    Methylene blue
  23. What are the results of mycobacterium smegmatis with a acid fast
    • staining
    • Red rods Blue cocci
  24. What is the primary stain in spore staining malchite green
  25. Cover the slide with malachite green a place slide atop a beaker of
    • boiling water
    • Spore staining
  26. Counter stain the stain with safranin
    Spore staining
  27. What is the counter stain in spore staining
  28. What is the result of spore staining escerichia coli and bacillus cereus
    Red rods and green spores
  29. To isolate a specific bacteria what method is used
    Pure culture
  30. Name two types of prue culture methods
    Streak plate method and Pour plate method
  31. What are the three types of gaseous requirements
    Aerobic, anaerobic and facultative
  32. what three methods are used to find the gaseous requirment of a
    • bacteria
    • Wright tube, thioglyollate and Petrie plate
  33. Slightly loosen the lid of a tube of thiogycollate broth and boil it
    • for 10 minutes, allowing the dissolved oxygen to be driven off.
    • Thioglycollate broth method
  34. Obtain a nutrient agar slant tubes, inoculate it with bacteria, place
    • a cotton plug into the test tube about one- fourth inch from the top
    • of the slant
    • Wright's tube method
  35. What acid is uesed in the wright's method
    Pytogallic acid
  36. What base is used in the wright's method
    10 percent NaOH
  37. Sterilize an inoculating loop and asepically streak one loopful of
    • bacteria onto one side of a Petri plate
    • Petri plate method
  38. What is the gaseous requirment of escherichia coli
    Facultative
  39. What is the gaseous requirment of clostridium sporogenes
    Anaerobic
  40. What is the gaseous requirment of sarcina lutea
    Aerobic
  41. What is the gaseous requirment of bacillus subtilis
    Aerobic
  42. Does escherichia coli grow in thioglycollate broth method
    Yes
  43. Does escherichia coli grow in
    • The wright's tube method
    • Yes
  44. Does escherichia coli grow in
    • Petri plate method
    • Yes
  45. Does clostridium sporogenes grow in thioglycollate broth method
    Yes
  46. Does clostridium sporogenes grow in
    • Wright's tube method
    • Yes
  47. Does clostridium sporogenes grow in petri plate method
    No
  48. Does sarcina lutea grow in triogycollate broth method
    No
  49. Does sarcina lutea grow in wright's method
    No
  50. Does sarcina lutea grow in petri plate method
    Yes
  51. Does bacillus subtilis grow in the thioglycollate broth method
    No
  52. Does bacillus subtilis grow in the wright's tube method
    No
  53. Does bacillus subtilis grow in the petri plate method
    Yes
  54. What are the cultural characteristics of bacillus cereus
    Flat, white, dry, motile, billowy, opaque
  55. What are the cultural characteristics of escherichia coli
    Raised, white, mucoid, motile, translucent
  56. What are the cultural characteristics of proteus vulgaris
    Flat, white, mucoid, motile, translucent
  57. What are the cultural characteristics of staphylococcus aureus
    Raised, white, mucoid, not motile, opaque
  58. What is hanging drop slide method used for
    To study motility
  59. What is the motility test agar method used for
    To test for motility
  60. What are the results of motility test on proteus vulgaris
    Motile
  61. What are the results of motility test on pseudomonas aeruginosa
    Motile
  62. What are the results of motility test on escherichia coli
    Motile
  63. What are the results of motility test on sarcina lutea
    Not motile
  64. In sugar fermentation the results of dextrose and bacillus subtillis
    Acid/no gas
  65. In sugar fermentation the results of dextrose and escherichia coli
    Acid/gas
  66. In sugar fermentation the results of dextrose and staphylococcus aureus
    Acid/no gas
  67. In sugar fermentation the results of dextrose and alcaligenes
    • viscolactis
    • Base/no gas
  68. In sugar fermentation the results of lactose and bacillus subtillis
    Base/no gas
  69. In sugar fermentation the results of lactose and escherichia coli
    Acid/gas
  70. In sugar fermentation the results of lactose and staghylococcus aureus
    Base/no gass
  71. In sugar fermentation the results of lactose and alcaligenes viscolactis
    Base/no gas
  72. In sugar fermentation the results of maltose and bacillus sbtilis
    Base/no gas
  73. In sugar fermentation the results of maltose and escherichia coli
    Acid/gas
  74. In sugar fermentation the results of maltose and staphylococcus aureus
    Base/no gas
  75. In sugar fermentation the results of maltose and alcaligenes visclactis
    Base/no gase
  76. In sugar fermentation the results of saccharose and bacillus subtillis
    Acid/no gas
  77. In sugar fermentation the results of saccharose and escherichia coli
    Base/no gas
  78. In sugar fermentation the results of saccharose and staphylococcus
    • aureus
    • Acid/no gas
  79. In sugar fermentation the results of saccharose and alcaligenes
    • viscolactis
    • Base/no gas
  80. In sugar fermentation the results of sucrose and bacillus subtilis
    Acid/no gas
  81. In sugar fermentation the results of sucrose and escherichia coli
    Base/no gas
  82. In sugar fermentation the results of sucrose and staphylococcus aureus
    Base/no gas
  83. In sugar fermentation the results of sucrose and alcaligenes viscolactis
    Base/no gas
  84. What is the reagent in the starch hydrolysis method
    Iodine
  85. What are the results of bacillus subtilis in the starch hydrolysis
    • method
    • Positive for starch hydrolace
  86. What are the results of staphylococcus aureus in the starch hydrolysis
    • method
    • Negative for starch hydrolysis
  87. What are the results bacillus subtilis in the urea hydrolysis method
    Negative no urease
  88. What are the results escherichia coli in the urea hydrolysis method
    Negative no urease
  89. What are the results proteus vulgaris in the urea hydrolysis method
    Positive produces urease
  90. What are the results staphylococcus aureus in the urea hydrolysis method
    Negative no urease
  91. The purpose of this exercise is to contrast this enzymatic ability
    • between various bacteria
    • Litmus milk method
  92. What color is the base in the litmus milk method
    Blue
  93. What color is the acid in the litmus milk method
    Red
  94. What are the results of escherichia coli
    • In the litmus milk method
    • Turned pink, positive for enzyme lactase
  95. What are the results of alcaligenes viscolactis In the litmus milk
    • method
    • Negative for enzyme lactase blue (produces ropey curd)
  96. What are the results of streptococcus lactis In the litmus milk method
    Positive for enzyme lactace (pink ring) round top (white clot bottom)
  97. What are the results of proteus vulgaris In the litmus milk method
    Negative for lactace (stayed blue) negative ropey curd
  98. What are the effects of catalase on staphylococcus aureus
    Positive
  99. What are the effects of catalase on saccharomyces cerevisiae
    Positive
  100. What are the effects of catalase on eschericia Coli
  101. What are the effects of catalase on streptococcus lactis
  102. What are the effects of hydrogen sulfide on proteus vulgaris
    • Positive for Sistine desulerace (enzyme)-enzyme, dark parcipitant
    • (lead sulfide)-product
  103. What are the effects of hydrogen sulfide on eschericia coli
    Negative for Sistine desulferase
  104. What do you add to nitrate broth when converting from nitrates to
    • nitrites
    • Sulfanilic acid and dimethyl alpha-napthalamine
  105. What is nitrate reduction
    • The enzymatic ability to convert nitrates to nitrites between various
    • bacteria
  106. What is the effect of nitrate reduction on proteus vulgaris
    Positive for nitratase-enzyme
  107. What is the effect of nitrate reduction on eschericia coli
    Positive for nitratase
  108. What is the effect of nitrate reduction on pseudomonas aeruginosa
    Positive for nitratase w/zinc
  109. What is the effect of nitrate reduction on staphylococcus aureus
    Positive for nitratase w/zinc

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