Although DNA polymerases require both a template and a primer, the following DNA was found to serve as a substrate for DNA polymerase in vitro in the absence of any additional DNA or RNA. Explain how this could happen and give the sequence of the product of this reaction. Note: dNTPs and DNA polymerase are supplied but no primase is around in this in vitro DNA synthesis reaction.
The blue region and the yellow region of this DNA are complementary to each other, thus the blue region can fold back to hybridize with the yellow region. The 3’-end of the blue region can be used as a primer (hence no need for primase) and be extended following a standard DNA synthesis process.