Midterm AA

Card Set Information

Author:
Anonymous
ID:
78220
Filename:
Midterm AA
Updated:
2011-04-07 22:57:44
Tags:
midterm
Folders:

Description:
midterm AA
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  1. ELISA:
    • (wells fixed w/ Ag)
    • Add P Serum
    • Wash
    • Secondary Ab: Anti-Ig
    • Wash
    • Substrate for Color Change
  2. Ag Capture ELISA:
    • (wells fixed w/ Ab)
    • Add P Ag
    • Wash
    • Ass known Ab + Conjugate
    • Wash
    • Substrate for color change
  3. Western Blot for Ag:
    • Electrophoresis
    • Blot
    • Probe w/ Ab + Conjugate
    • Wash
    • Add Substrate (color or radioactive)
  4. Western Blot of Ab:
    • Electrophoresis
    • Blot
    • Probe w/ Anti-Ig
    • Wash
    • Add Substrate (color or radioactive)

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