IS 352 Test 2

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IS 352 Test 2
2011-04-11 13:05:51
chromatography acids bases carbohydrates lipids protiens

IS 352 Test 2 Review
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  1. Chromatographty
    method that is used to separate the parts of a mixture
  2. Chromatogram
    the visual output of the chromatography
  3. solute
    smaller amount, is dissolved
  4. Solvent
    largest amount, does the dissolving
  5. capillary action
    the movement of a liquid along the surface of a solid caused by the attraction of molecules of the liquid to the molecules of the solid
  6. adhesion
    when an attractive force exists between two unlike materials, such as a liquid and a solid
  7. cohesion
    the mutual attractive force that exists between like molecules
  8. mixture
    a combination of 2 or more substances that are physically mixed - parts keep their own characteristics
  9. How did water move through the coffee filter?
    The movement starts as adhesion, where the water particles are attracted to the paper particles, then as cohesion, where the water trails along the path by a strong attraction between the water molecules themselves.
  10. Activity: Chromatographic Circles
    The water moved from the middle of the coffee filter to the edges of the coffee filter first by adhesion then cohesion. As the water moved it separated the color mixture that made up the different black markers. Each marker company uses a different mixture of colors to create black. Some of the circles showed blue, green, and brown colors. Some didn't change at all. The black marker is a solution and the chemicals are the pigments. Some are water soluble and some are not. The further out the water traveled the easier it is to break apart the mixture. This is why you will see the brighter colors towards the outside of the coffee filter.
  11. Properties of Acids
    • Sour taste i.e. citrus fruit, vinegar
    • If dissolved in water (aqueous solution) (HCl) added to metals, metal will dissolve and hydrogen gas is released (corrosive)
    • If aqueous solution (HCl) it will change the color of litmus paper from blue to red
    • When acids react with bases they form salt and water
    • Acid will neutralize a base
    • Electrolytes - will conduct electricity when dissolved in water - has free ions
    • pH of <7
  12. Properties of Bases
    • bitter taste
    • feel slippery i.e. bar of soap
    • in aqueous solutions will turn litmus from red to blue
    • react with an acid to form salt and water
    • electrolyes - has free ions
    • very corrosive
  13. Base
    • any substance that is a proton acceptor when it is dissolved in water
    • will produce hydroxide ions
    • generally consists of a metal and one or more hydroxide groups
  14. Acid
    • any substance that is a proton donor when it's dissolved in water
    • positive hydrogen ions quickly surrounded by a water molecule end up with hydronium ion => H30 or H+
  15. Hydronium ion
    a water molecule with an extra hydrogen ion attached to it (H2O + H+=H3O+). It's usually used to determine the acidity of a chemical compound.
  16. Hydroxide Ion
    consists of an oxygen atom and a hydrogen atom that are covalently bonded to each other. the net charge of a hydroxide ion is -1. In chemical notation, a hydroxide ion is indicated as OH-. Compounds that contain hydroxides usually use ionic bonds.
  17. Salt
    any ionic compound formed from the reactions between a base and an acid called a neutralization reaction between the hydronium ions and the hydroxide ions. Does not taste bitter or sour, no reaction with litmus, soluble in water
  18. Neutralization reaction
    when a strong acid and a strong base solution are mixed, a neutralization reaction occurs, and the products do not have characteristics of either acids or bases. Instead, a neutral salt and water are formed.
  19. pH scale range
    • a measure of the hydronium ion concentration of a solution
    • 0-14
    • 7 neutral
    • >7 = base
    • <7 = acid
  20. What is the buffer system?
    a weak acid and a weak base that work together to counter shifts in pH
  21. What is carbonic acid?
    a weak acid with the formula H2CO3. It is formed in small amounts when carbon dioxide is dissolved in water, and it is usually only found in solution.
  22. What is acid rain?
    Rain with a pH of 5.5-4.0. In the atmosphere the sulfuric acid is much stronger than the carbonic acid.
  23. What causes acid rain?
    20 million tons of sulfur dioxide is released into the atmosphere due to the burning of fossil fuels, petroleum, coal and natural gas.
  24. What effects does acid rain have?
    • Forests
    • destroys leaves
    • leaches nutrients and causes trees to go into distress
    • Lakes
    • fish populations can't survive
    • Building materials
    • acid will eat away marble and limestone
    • Crops
    • yield and quality has declined due to acid rain
  25. Solutions to prevent acid rain:
    • carpool
    • solar energy
    • find other sources of energy such as wind energy
  26. Activity: Making an Indicator
    Using red cabbage leaves and rubbing alcohol, we mad an indicator. When mixed in ammonia the solution turned green, and when mixed in vinegar the solution turned pink. All but baking soda which is a base, tap water which is usually neutral and distilled water which is neutral were acids.
  27. Activity: Magic of Your Words
    When blowing through the straw into the phenol red and water mixture the accumulation of CO2 from your breath causes a decrease of pH by combining with water to create carbonic acid. Releases hydrogen ions, which react with phenol red causing the mixture to turn yellow.
  28. Phenol Red
    a type of indicator like litmus
  29. Purposes of carbohydrates
    • have a structural role - provides a certain shape to a cell
    • serve as an immediate form of energy which can be transported to other places in the body
    • stored energy
  30. Carbohydrates: 3 classes and examples
    • 1. monosaccharide
    • a. simple sugars
    • b. 1 sugar unit
    • c. soluble in water
    • d. sweet tasting
    • e. glucose, fructose, galactose, ribose, deoxyribose
    • 2. disaccharide
    • a. short chain of only 2 monosaccharides
    • b. dehydration synthesis - a water molecule is eliminated which allows 2 monosaccharides to combine
    • c. sucrose, lactose, maltose
    • 3. polysaccharide
    • a. branching chains of monosaccharides
    • b. complex carbs
    • c. cellulose - (walls of plants) main food source for herbivores, termites, fiber for humans, no calories
    • d. starch - (used to store energy in plants) during digestion turns to glucose when body has more than it needs it turns to glycogen
    • e. glycogen - storage form of glucose in animal tissue - when used turns to glucose for immediate energy
    • f. chitin - main structural materials in exoskeletons
  31. Lipids
    • greasy, oily hydrocarbon linked together by non-polar covalent bonds, very hydrophobic
    • energy storage, cell membrane development and serves as a component of hormones and vitamins in the body
  32. Lipids: 4 types and examples
    • 1. true fats
    • a. solid - saturated fat - get from animals, at room temp. this fat tends to be solid
    • b. liquid - unsaturated fat - plant fats, liquid at room temp. i.e. olive oil and corn oil
    • 2. phospholipids
    • a. water insoluble, organic molecule
    • b. main component of cell membrane (phospholipid bilayer)
    • c. made up of phosphate group - 2 fatty acid tails and a hydrophilic head
    • 3. steroid
    • a. found in cell membranes cholesterol
    • 4. waxes
    • a. fatty acid chains
    • b. firm yet pliable i.e. cherry covering
    • c. cuticle (waxy coating) on fruit that helps it preserve water
    • d. lubricates skin and hair on animals, and coating on duck feathers
  33. Proteins
    • A polymer made of monomers known as amino acids - the building blocks for proteins
    • messenger, transport, structural, enzyme
  34. Structural formula for an amino acid:
    • see review
    • central carbon with an R group that determines the type of amino acid
  35. What type of bond joins amino acids together?
    peptide bond - small chain covalent bond joins 1 amino acid to another
  36. What are essential amino acids?
    20 different amino acids differentiated by R group. R group determines type of amino acid. 8 are essential amino acids - our body cannot make them, we get them from what we eat
  37. Nucleic acids
    • molecules that store and transfer info within a cell, originally found in nucleus
    • DNA - deoxyribonucleic acid
    • RNA - ribonucleic acid
  38. Nucleic Acid structure
    • DNA - double helix
    • RNA - single chain
  39. DNA v. RNA
    3 differences:
    • 1. Structure
    • DNA - double helix
    • RNA - single chain
    • 2. Sugars
    • DNA - deoxyribose
    • RNA - ribose
    • 3. Bases
    • DNA - thymine
    • RNA - uracil
  40. DNA v. RNA:
    Base Pair Rule
    • Nitrogenous bases
    • Adenine
    • Thymine
    • Cytosine
    • Guanine
    • Uracil

    • DNA Pairings - hydrogen bond between the pairs
    • AT CG GC TA

    • RNA Pairings
    • AU CG GC UA
  41. Activity: Food Testing
    Iodine & Benedict's Reagent solution - What does each of these test for?
    • Iodine tests for starch
    • Benedict's Reagent tests for simple sugars
  42. Amylase
    an enzyme found in saliva that breaks down starch and sugars into simple sugars
  43. Top 4 elements that make up the human body:
    • Oxygen - 65%
    • Carbon - 18%
    • Hydrogen - 10%
    • Nitrogen - 3%
  44. How much of the body's weight is water?
  45. What are the human nutritional requirements?
    • Carbohydrates - 55-60%
    • Lipids - 30%
    • Proteins - 15-20%
    • 20 total proteins - 8 essential
  46. Vitamin
    • an organic substance, not an energy source, and will help carry out cellular metabolism
    • 13 vitamins - 2 categories
    • water soluble - need to be replenished daily
    • fat soluble - stored in fatty tissue, don't need to be replenished as often
  47. Mineral
    an inorganic substance needed for normal growth and maintenance
  48. Incomplete v. Complete Digestive Sytem
    • Incomplete - a sack-like gut with only one opening, same mouth and anus i.e. flat worm
    • Complete - one long tube with two openings, mouth on one end and anus on the other, food moves in one direction i.e. human
  49. Function of the digestive system:
    To break down food into smaller parts so the body can use them for energy and cell nourishment.
  50. Trace food through the digestive system
    • Mouth (teeth - mechanical breakdown, saliva - chemical breakdown) food leaves as bolus ->
    • Pharnyx - a thick-walled muscular tube, opening to the esophagus ->
    • Esophagus - bolus moves down the esophagus to the stomach ->
    • Stomach - bolus spends 2-6 hours here - leaves as chyme and moves to the small intestine ->
    • Small Intestine - chyme moves into the small intestine which is broken up into 3 sections, duodenum, jejunum & ileum. Whatever is left moves on to the large intestine ->
    • Large Intestine - where the water is removed and reabsorbed to prevent dehydration, feces is formed, moves to the rectum and out the anus ->
    • Rectum ->
    • Anus
  51. Which enzyme digests what:
    Salivary amylase & Pepsin
    • Salivary amylase - breaks down starch and sugars into simple sugars
    • Pepsin - starts the breakdown of protein, breaks peptide bonds
  52. Bolus
    a soft ball of food that has been broken down in the mouth and will move through the esophagus to the stomach
  53. Chyme
    a semi-fluid mixture of partially digested food after it has been broken down by gastric acid in the stomach and is moving to the small intestine
  54. Peristalsis
    a wave-like movement of the esophagus that moves the bolus to the stomach
  55. Bulk
    indigestible material in the large intestine
  56. Fiber
    indigestible material of cellulose
  57. Villi
    finger-like protrusions that line the small intestine and increase the surface area of the small intestine for better absorption of nutrients
  58. Microvilli
    smaller finger-like protrusions that line the villi
  59. Where does the actual absorption of nutrients take place?
    small intestine
  60. What is the function of the liver?
    Produce and secrete bile that helps digest fats in the small intestine
  61. What is the function of the pancreas?
    • completes the job of breaking down protein, carbohydrates, and fats using digestive juices of pancreas combined with juices from the intestines
    • Secretes hormones that affect the level of sugar in the blood
    • Produces chemicals that neutralize stomach acids that pass from the stomach into the small intestine by using substances in pancreatic juice
  62. What is the function of the gall bladder?
    The gall bladder stores bile secreted by the liver
  63. What 3 minerals is the American diet deficient in?
    Calcium, Iron & Iodine
  64. Vitamin Deficient Diseases
    • Scurvy - Vitamin C deficient
    • Beriberi - Vitamin B1 deficient
    • Pellagra - Vitamin B3 deficient
    • Blindness - Vitamin A deficient
    • Rickets - Vitamin D deficient