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What genus does a catalase rxn identify?
Staph vs strep
What is a positive catalase rxn and what genus is it?
Why does an organism have catalase?
To neutralize txic forms of O2
What organism does a coagulase rxn identify?
Pathogenic( S. aureus) vs. Nonpathogenic staphylococci
What ia a positive coagulase rxn?
Clotting in rabbit plasma(S. aureus)
What is coagulases benefit to bacteria?
An organism is encased in a clot that helps it avoid phagocytosis
What is a positive result for starch hydrolysis?
An organism with a halo around it on the media
What enzyme is used in statch hydrolysis?
What reagent is used in starch hydrolysis?
What organism was used in the starch hydrolysis experiment?
What were the urea experiment results?
- Orange: neg.
- Hot pink: pos.
What was the chemical reaction that happened in the urea experiment?
What enzyme did the urea experiment utilize?
What organism was used in the urea experiment?
What were the results for the TTC motility test?
- All purple: true motility
- Purple by stab only: Brownian movement
What organisms were used in the TTC motility experiment?
- E. coli: true motility
- K. pneumoniae: Brownian movement
What is deanimation?
An amine group (NH2) on a molecule removed and an amino acid is degraded
What were the results for the deanimation experiment?
Presence of black in the slant media was pos.
What is decarboxylation?
Removing the carboxal group from the amino acid
What is Bromcresol?
A pH indicator
What were the results for the decarboxylation experiment?
- Purple: 6.8> alkaline
- Yellow: 5.2< acidic
What were the results for the SIM experiment?
- Yellow: tryptophan breakdown=indole production
- Black: reducion of sulfur (H2S)
- Turbidness: true or brownian movement
What were the results for the Bile esculin test?
Brown/black color in over half of the medium was positive for esculin hydrolyzation
What genus does the Bile esculin experiment identify?
Group D strep
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