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What is Sheep Blood Agar used for?
Works well for growing strep
What agar will we be using most in this class?
What does McKonkey agar only growt?
Grows only gram positive bacteria
What is Chocolate agar made of and what is it used to detect?
- Cooked RBC
- Good for growing gonorrhea
What is a spore?
- A package of DNA within a cells that lives after the bacteria dies.
- Forms new bacteria
- Gram Stains Clear
Is the Genus Bacillus:
Anaerobic or aerobic?
Catalase positive or negative?
Where is it found?
- In the soil
How does bacillus anthracis infect animals?
Through the soil
What is cutaneous anthrax and how do you get it?
Surface anthrax, by getting cow poo on your skin
What is pulmonary anthrax?
- Terrorist attacks- gets into lungs (most deadly)
- Can also get from working with animals
How is anthrax treated and is there a vaccine?
- Ciproflaxin (top choice) or Penicillin
- There is a vaccine- a shot given 6 times a year and a half with yearly boosters
What is done with animals that die from anthrax?
They must be incinerated
What does bacillus cereus cause?
The 24 hour flu from undercooked meats
Is genus Clostridium,
Anaerobic or aerobic
Catalase negative or positive
Where is is found?
- In the soil
What causes gangrene?
What are the 2 forms of gas gangrene?
- Anaerobic cellulitis- very localized
- True myonecrosis- gets out of control (spreads)
How is gangrene treated?
Amputation or Overwhelm with Oxygen
What causes Clostridium defficile (C. def)?
Psuedomembranous colitis- kills all the good bacteria by giving too many antibiotics
What are the side effects of C. def and what is the treatment?
- Severe diarrhea
- Treated with Flagyl
What is the incubation time for tetanus?
What happens when a person is infected with tetanus?
- It attacks the spinal cord (ventral horns- motor side)
- Causes muscles to contract all the time
How does a person infected with tetanus die?
Asphyxiation, due to the diaphragm constantly being contracted
What is the treatment and prevention of tetanus?
- Tetanus Immune globulin (TIG)- give antibodies
- Prevention- vaccine with toxin (Td) Antigens
What causes Clostridium perfringens, Subtype A, and what are the side effects?
- Food poisoning- meat, fish
- Diarrhea for 16 hours
What causes Clostridium botulinum?
- Food- canning food, not processing properly
- Spores get into intestines, absorbed into blood stream, produces toxins that don't allow the muscles to contract
What are the side effects and incubation period of botulism?
- Toxins that look like acetylcoylene get into the nerve and don't allow the muscles to contract, causes asphyxiation
- 17-72 hours
What is the treatment of botulism?
- History of what is eaten is very important
- Lab work takes too long
- If caught, pump stomach, Flagyl, and Antitoxin
What would you like to do?
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