Micro Lab Practical

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AJWLion
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82473
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Micro Lab Practical
Updated:
2011-04-28 09:42:12
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Lab Practical
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Lab Practical
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  1. What is indicated by the blue color of this tube of litmus milk media, inoculated with Pseudomonas aeruginosa, compared with the purple negative control tube?

    alkalinization of media by ammonia production
  2. What is indicated by the cleared, purple zone on the top of this Bacillus cereus culture in this litmus milk tube?
  3. What is indicated by the pink region in this tube of litmus milk inoculated with Escherichia coli?
  4. The pattern of hemolysis observed around these colonies of Staphlococcus epidermidis on this blood agar plate is
  5. The pattern of hemolysis observed around colonies of Staphlococcus aureus on this blood agar plate is
  6. The pattern of hemolysis observed around these colonies of Echerichia coli on this blood agar plate is
  7. Shape 1
  8. Shape 2
  9. Shape 3
  10. Shape 4
  11. Shape 5
  12. Shape 6
  13. Shape 7
  14. What genus of bacterium (round cells, catalase positive) is able to grow readily on mannitol salt agar, which is 7.5% NaCl? This particular species did not turn the media yellow, as some do.
  15. What is probable about a bacterium, such as this, which did not grow on a plate of Eosin Methylene Blue (EMB) agar?
  16. This bacterium, which grew readily on EMB agar but has pale pink colonies,
  17. A Gram-negative rod-shaped bacterium which grows well on Eosin Methylene Blue agar, and whose colonies are dark blue with a metallic green sheen due to lactose fermentation (with acid and gas) is most likely...
  18. This pathogenic bacterium has round, clustered, Gram-positive cells and is catalase positive. It grew well on Mannitol Salt Agar and turned it yellow. It is likely...
  19. What is likely about this bacterium, which was inoculated by stabbing vertically into this tube of thioglycolate medium, and grew only at the top?
  20. What is likely about this bacterium, which was inoculated by stabbing vertically into this tube of thioglycolate medium, and grew throughout the stab line?
  21. What can be concluded about this blue, coccus-shaped bacterium which as stained with hot carbol fuchsin, destained with acid-alcohol, and counterstained with methylene blue?
  22. What genus might this pink, rod-shaped bacterium which as stained with hot carbol fuchsin, destained with acid-alcohol, and counterstained with methylene blue?
  23. Types of Stains
  24. This teardrop-shaped protozoan cell, which has two nuclei and several flagella (not visible) infects the intestine of mammals.
    Giardia lamblia
  25. These small, purple-stained Apicomplexan protozoan cells, infecting erythrocytes, belong to the genus ____________, and are vectored by Anopheles mosquitoes in the tropics.
  26. These elongate protozoans, bearing a single terminal flagella, infect the blood and nervous system, eventually causing coma and death, are vectored by Tsetse flies.
  27. This large, ovoid, purple-stained ciliate protozoan genus causes infections of the colon and diarrhoea.
  28. What enzyme does this substance, 3% hydrogen peroxide, test for in bacterial cultures? Bubbling of the culture indicates a positive test.
  29. This plate was inoculated with an agar suspension of Esherichia coli B and material from sewage which passes through a 0.2 um pore size filter. The clear zones in the lawn of bacterial growth represent
  30. What can be concluded about this red-stained microbe, stained with Hucker’s (Crystal) Violet, fixed with iodine,
    destained with ethanol, and counter-stained with saffranin?
  31. What can be concluded about this blue-stained microbe, stained with Hucker’s (Crystal) Violet, fixed with iodine,
    destained with ethanol, and counter-stained with saffranin?
  32. How was this bacterium, with clear cells on a dark background, stained?
  33. What kind of blood cell is this rather small one with the large purple nucleus and very thin region of surrounding cytoplasm? They function in the adaptive immune system.
  34. What kind of cell is this moderate-sized white blood cell with a purple, segmented nucleus?
  35. What is indicated by the yellow color in this tube of glucose phenol red media?
  36. This aerobic bacterium, which was stained with hot malachite green and counter-stained with saffranin, has _______and probably belongs to the genus__________.
  37. What is gas is indicated by the bubble in this Durham tube, produced by soil bacteria growing anaerobically in this tube of nitrate broth, which contains no fermentable sugars?
  38. What product was produced by this culture of E. coli growing anaerobically in this tube of nitrate broth, which contains no fermentable sugars, as demonstrated by the red colour after addition of sulfanilic acid and NEDA reagents to the sample? No zinc was added.
  39. What is gas is indicated by the bubble in this Durham
    tube, produced by soil bacteria growing anaerobically in this tube of nitrate broth, which contains no fermentable sugars?
  40. What product was produced by this culture of E. coli growing anaerobically in this tube of nitrate broth, which contains no fermentable sugars, as demonstrated by the red colour after addition of sulfanilic acid and NEDA reagents to the sample? No zinc was added.
  41. How do you calculate CFL?
  42. How do you calculate PFL?

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