Lab Practical 2

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Lab Practical 2
2011-04-28 22:21:12
lab bio

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  1. How to calculate magnification of microscope
    Piece you put your eye to (10x) multiplied by the objective lens power
  2. Surface area of a block
    Area of one side multiplied by the number of sides
  3. Volume of a block
  4. High SA:Vol. ratio
    less productivity
  5. Low SA:Vol. ratio
    high productivity
  6. Diffusion
    high concentration to low concentration
  7. Osmosis
    diffusion of water or other solvent molecules through semipermeable membrane
  8. Dialysis
    diffusion of a dissolved substance, or solute, through a semipermeable membrane
  9. How is the molecular size of a gas related to how far it travels?
    the lighter, the farther
  10. How does a change in temperature affect the rate of movement of a gas?
    Higher temperature= higher rate of diffusion
  11. In Osmosis, is the solute or solvent moving?
  12. If a solution one is
    hypotonic to solution two, the second solution must be ______ to the first.
  13. If a plant wilts, what is
    happening within the cells to cause this appearance?
    Plasmolysis (water exits the cell via osmosis)
  14. What is plasmolysis and how
    is it related to osmosis?
    Water exiting the plant; exits by osmosis
  15. How are the terms
    hypertonic and hypotonic related to plasmolysis?
    a plant in a hypertonic solution will wilt because the cytoplasm inside the cells are hypotonic
  16. Dialysis tests:
    Inside before
    • Tests for Cl-
    • add 3 drops of AgNO3
    • Milky white= yes
  17. Dialysis test:
    Outside before
    • Tests for SO42-
    • add 3 drops of BaSO4
    • white precipitate= yes
  18. Dialysis test:
    Inside after
    • Tests for protein
    • dip an Albustix reagent strip in each tube
    • green/blue-green= yes
  19. Dialysis test:
    Outside after
    • Tests for starch
    • add 3 drops of Lugol's (iodine)
    • dark blue= yes
  20. How do the absorbance
    characteristics of chlorophyll differ or change across the spectrum?
    It does not absorb a lot from 500-575nm (green)
  21. How did you produce
    absorbance data for chlorophyll?
    How did you obtain your chlorophyll extract?
    Using a spectrophotometer; put spinach leaf in ethanol and heat it up
  22. Why did you initially make
    readings as percent of transmission rather than as absorbance during the chlorophyll extracting experiment?
    to make sure there was enough chlorophyll
  23. During the chlorophyll extracting experiment you
    were required to get a transmittance with your chlorophyll solution of between
    65% and 85%. Would the concentration be too high or too low if you acquired a transmittance of 52%? Why?
    Too high because there is some blue being transmitted
  24. In the experiment with the light and Elodea in a test tube, what was the role of sodium
    bicarbonate in the large test tube with the Elodea?
    provide CO2
  25. What two tests could be
    used to detect the presence of CO2 in expired air?
    yeast fermentation and Elodea in a test tube with light
  26. How could you determine if a plant used or produced CO2 during photosynthesis?
    If the orange water turned pink it used CO2
  27. In order to determine if fermentation actually occurred, what tests were performed to detect the
    • adding CO2 to Barium hydroxide to get a white precipitate (alcohol present)
    • adding sodium iodide and sodium hydroxide to the solution to get a yellow precipitate (alcohol present)
  28. What metabolic process was being investigated using the hamburger experiment? Where in the hamburger meat did this process happen?
    Citric Acid Cycle; mitochondria
  29. What is the relationship of succinic acid dehydrogenase to the hamburger experiment?
    convert succinic acid to fumeric acid
  30. In this experiment what is/are the: Substrate, oxidation-reduction reaction(s), electron acceptor,
    Co-enzyme, role of methylene blue, role of Malonic acid
    • Substrate: succinic acid
    • Oxidation-Reduction rxn: succinc acid removes hydrogen
    • Electron acceptor: FAD
    • Co-enzyme: FAD
    • Methylene blue: enzyme
    • Malonic acid: inhibitor
  31. Is the process beingevaluated in the hamburger experiment anaerobic or aerobic?