Lab Practical 2
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How to calculate magnification of microscope
Piece you put your eye to (10x) multiplied by the objective lens power
Surface area of a block
Area of one side multiplied by the number of sides
Volume of a block
High SA:Vol. ratio
Low SA:Vol. ratio
high concentration to low concentration
diffusion of water or other solvent molecules through semipermeable membrane
diffusion of a dissolved substance, or solute, through a semipermeable membrane
How is the molecular size of a gas related to how far it travels?
the lighter, the farther
How does a change in temperature affect the rate of movement of a gas?
Higher temperature= higher rate of diffusion
In Osmosis, is the solute or solvent moving?
If a solution one is
hypotonic to solution two, the second solution must be ______ to the first.
If a plant wilts, what is
happening within the cells to cause this appearance?
Plasmolysis (water exits the cell via osmosis)
What is plasmolysis and how
is it related to osmosis?
Water exiting the plant; exits by osmosis
How are the terms
hypertonic and hypotonic related to plasmolysis?
a plant in a hypertonic solution will wilt because the cytoplasm inside the cells are hypotonic
- Tests for Cl-
- add 3 drops of AgNO3 Milky white= yes
- Tests for SO42-
- add 3 drops of BaSO4
- white precipitate= yes
- Tests for protein
- dip an Albustix reagent strip in each tube
- green/blue-green= yes
- Tests for starch
- add 3 drops of Lugol's (iodine)
- dark blue= yes
How do the absorbance
characteristics of chlorophyll differ or change across the spectrum?
It does not absorb a lot from 500-575nm (green)
How did you produce
absorbance data for chlorophyll?
How did you obtain your chlorophyll extract?
Using a spectrophotometer; put spinach leaf in ethanol and heat it up
Why did you initially make
readings as percent of transmission rather than as absorbance during the chlorophyll extracting experiment?
to make sure there was enough chlorophyll
During the chlorophyll extracting experiment you
were required to get a transmittance with your chlorophyll solution of between
65% and 85%. Would the concentration be too high or too low if you acquired a transmittance of 52%? Why?
Too high because there is some blue being transmitted
In the experiment with the light and Elodea in a test tube, what was the role of sodium
bicarbonate in the large test tube with the Elodea?
What two tests could be
used to detect the presence of CO2 in expired air?
yeast fermentation and Elodea in a test tube with light
How could you determine if a plant used or produced CO2 during photosynthesis?
If the orange water turned pink it used CO2
In order to determine if fermentation actually occurred, what tests were performed to detect the
- adding CO2 to Barium hydroxide to get a white precipitate (alcohol present)
- adding sodium iodide and sodium hydroxide to the solution to get a yellow precipitate (alcohol present)
What metabolic process was being investigated using the hamburger experiment? Where in the hamburger meat did this process happen?
Citric Acid Cycle; mitochondria
What is the relationship of succinic acid dehydrogenase to the hamburger experiment?
convert succinic acid to fumeric acid
In this experiment what is/are the: Substrate, oxidation-reduction reaction(s), electron acceptor,
Co-enzyme, role of methylene blue, role of Malonic acid
- Substrate: succinic acid
- Oxidation-Reduction rxn: succinc acid removes hydrogen
- Electron acceptor: FAD
- Co-enzyme: FAD
- Methylene blue: enzyme
- Malonic acid: inhibitor
Is the process beingevaluated in the hamburger experiment anaerobic or aerobic?
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