MicroBio

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Author:
chiroclown
ID:
82918
Filename:
MicroBio
Updated:
2011-05-01 00:46:46
Tags:
MicroBiology Test
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Description:
Chapters 1-8 (Supposedly
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  1. Perfect ratio for surface are and volume
    • L2/ L3= 1.2
    • SA/V
  2. Substrate
    Cell extracts energe and creates an external product from the chemical reaction
  3. Open System
    Anything alive
  4. Rate of division
    When a cell decides to make a copy of itself, how fast does it occur?
  5. Rate of division in human cells.
    25 x 1012
  6. Growth Curve Equation
    Nf = Ni x 2n
  7. Stages of growth curve
    • Lag
    • Log
    • Stationary
    • Death
  8. n in growth curve
    hours/ division rate
  9. Growth curve with very little/ no lag
    • Most Virulent
    • Thermodynamically working at maximum speed
  10. Growth curve with long/ slow lag
    Less virulent
  11. Process for virus
    • Protein finds a recpetor
    • Virus is received or denited
    • Virus is copied by cell upon entrance
    • Exits out to envirnment
  12. Can exist outside a cell and influences the process.
    Endogenous
    Bacteria
  13. Exists in cell and influences the product and cell.
    Indogenous
    Virus
  14. Subclinical stage of growth rate
    Lag Time
  15. Koch Postulate
    • Four criteria to establish causual relationship between microbe and disease
    • 1) isolate organism from person
    • 2) identify the isolated organism
    • 3) test on a healthy animale
    • 4) repeat to determine if organism is indeed a pathogen
  16. Fundamental pieces of cell
    • Carbs
    • proteins
    • lipids
    • nucleic acid
  17. Amino Acid
    H-C-(NH2)(COOH)-R
  18. Virus 'Pads'
    Proteins
  19. Compatible Virus
    • If the receptor on a potential host is a proteind then it invites the virus protein in.
    • Shapes looking for each other
  20. Treatment for Viruses
    attempt to hide the receptor so the virus can't find its 'match'
  21. Enzymes in virus
    • catalyst
    • more effective enzymes result in rapid cell copying
  22. Serology Test
    • 1) Antogens- place on slide and wait for chemical reactions
    • 2) antibodies
  23. Procaryote
    • 4 billion years ago
    • Simple
    • Anaerobic
    • No nucleus
    • Cytoplast is work central
    • Ribosum
    • Very durable cell wall
    • Inorganic Chemistry
  24. Eucaryote
    • 2 Billion years ago
    • Complex
    • Nucleus
    • Ribosom
    • Mitochondria
    • Chloroplasts
    • Aerobic
    • Organic Chemistry
  25. Organells
    • specialized subunit within a eucaryotic cell with a specific function
    • usually separately enclosed within its own lipid bilayer
  26. Current O2 levels in atmosphere
    30-39%
  27. Glycolysis
    • metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+.
    • The free energy released in this process forms the high-energy compounds ATP (adenosine triphosphate)
  28. RBC
    • Most primative cell
    • No nucleus
    • not sophisticated
    • glucose in ---> lactate out
  29. Delta G
    Potential free energy in glucose
  30. Freed energy from glycolisis
    Kinetic Energy
  31. To kill cells in large numbers the the target is....
    Enzymes in metabolic state
  32. Invagination in membrane
    • increases surface area and allows the cells to work easier
    • Cannot have more volume than SA to move goods in and out
  33. Pilus
    extension from cell
  34. Lipids and Gram Stain
    Lipids = Gram Negative
  35. Blood Agar Plates
    7% sheep RBC
  36. Crystal Violet
    Iodine
    Alcohol
    Safarin (counter stain)
  37. Which of the followin will lose color in Gram Stain?
    Gram Negative in Alcohol
  38. To stain for TB?
    Acid Fast Stain
  39. Nf
    • Where cells meet time
    • B
  40. Ni
    • Start of # of cells
    • A
    • germs get going
  41. n
    • Number of cells
    • log
    • D
    • Symptoms present
  42. Lag
    • Cells looking at DNA to determine next steps...
    • C
  43. Stagnate
    • Top of curve
    • E
    • Treatment
  44. Decline of curve
    • death of cells
    • F
    • Recovery
  45. Microbiology studies/ encompasses
    • Bacteria
    • Viruses
    • Fungi
    • Protozoa
    • Algae
    • Helminths
  46. Recombinant DNA
    Technology that allows the deliberate altering if DNA
  47. Morphology
    Structure
  48. Physiology
    Function
  49. Genetics
    Inheritence
  50. Glycocalyx
    functions in attachment to other cells as receptors
  51. Gram Stain
    Delineates two different groups of bacteria
  52. Gram Positive
    Looks like an open faced sandwich
  53. Gram Negative
    3 layer sandwich
  54. Pyruvic acid
    • key intersection in several metabolic pathways.
    • output of the anaerobic metabolism of glucose known as glycolysis

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