Bio Ch 20

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Author:
Anonymous
ID:
84034
Filename:
Bio Ch 20
Updated:
2011-05-05 17:51:08
Tags:
FML
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Description:
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  1. Functions of DNA cloning
    • –Make many copies of a gene
    • •Insert in another target organism
    • –Harvest gene product (protein) from bacteria
    • •Use protein for practical application
    • •Ex. Human insulin production by yeast
  2. Restriction Enzymes
    • •Used to cut DNA molecules
    • –Allow DNA from two sources to be combine
    • •Recognize specific nucleotide sequence
    • –Restriction site of DNA is cut
    • –Produces restriction fragments
    • •Fragments have “sticky” ends
    • –DNA cut with same enzyme has the same complementary ends for reattachment
    • •Found in bacteria as anti-viral defense
  3. General Procedure for Gene Cloning
    • 1)Isolation of plasmid and gene source DNA
    • -Bacterial plasmid used as vector. Gene source DNA (ex. gene from animal cell)
    • 2) Cut Cut both DNA samples with same enzyme
    • 3) Mix the plasmid and target DNA
    • 4 )Mix new plasmid with bacterial cells
    • 5) CLoning of genes via bacterial

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