Microbiology

Card Set Information

Author:
Johng9999
ID:
89643
Filename:
Microbiology
Updated:
2011-06-09 06:34:41
Tags:
Lab
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Description:
Agar Plates
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  1. PEA
    • Gram positive selective.
    • Gram negative - No growth
  2. MSA
    • Salt tolerant Staphlococcus
    • S. aureus will show yellow
    • Growth with no color change - Other staph
    • Gram neg no growth
  3. BAP
    • Nonselective - Gram pos and neg
    • Hemolysis

    • Beta - Complete hemolysis
    • Alpha - Partial hemolysis
    • Gamma - No hemolysis
  4. EMB
    Eosin Methlyne Blue Agar
    • Gram neg - Differentiates between LF (coliforms) and NLF.
    • Gram Pos - No growth
    • Gram Neg LF - Purple/Green sheen
    • Gram Neg NLF - Pink
  5. MAC
    Maconkey
    • Same as EMB
    • Gram Pos - No growth
    • Gram Neg LF - Dark Pink (DARK)
    • Gram Neg NLF - Colorless
  6. HE
    Hektoen Enteric Agar
    • Selective and Differential for
    • Salmonella and Shigella
    • Gram Pos - No growth
    • Gram Neg - VARIES
    • Salmonella and Shigella - Blue/Green
    • H2s - Black colonies
  7. Centrimide Agar
    • Selective for Psudomonas auruginosa
    • If Psudomonas auruginosa present growth present - Green pigment is called Pyocyanin - Illuminates under UV light
  8. E-Coli
    Gram Negative Bacteria
  9. Staph aureus
    Gram Positive
  10. Bacillus
    Gram Positive Spore Forming
  11. Iodine is what part of Gram Stain?
    Mordant
  12. Residurin Indicator turns what color when exposed to O2?
    Turns Pink

    Methaline Blue turns Blue
  13. Postively Charged Dyes
    • Saffarin
    • Crystal Violet
    • Methaline Blue
  14. Negatively Charged Dye(s)
    Nigrosin
  15. What is a faucaltive anarobe
    Does not need oxygen to survive but preffers it.

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