micro lab exam 2

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sapperwife
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micro lab exam 2
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2011-07-23 00:03:33
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lab exam 2
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  1. reason to learn about microbiota
    #1
    to gain an understanding of different bacteria at specific body sites. which provide greater insight to possible infections that result from injury
  2. reason to learn about microbiota #2
    to gain knowledge of the possible source and significance of bacteria isolated from clinical infections.
  3. reason to learn about microbiota #3
    to gain knowledge of consequences of overgrowth of those bacteria normally absent at a specific body site.
  4. reason to learn about microbiota #4
    to be aware of bacteria's play in stimulating the host immune system
  5. what constitutes a possitive reaction with 2O2?
    bubbles of oxygen gas is a posstive reaction
  6. gram stain purpose of each reagent #1
    primary stain cyrstal violet interacts with the peptidglycan in the cell wall
  7. gram stain purpose of each reagent #2
    the mordant iodine increases the interaction of the cyrstal violet and the cell wall
  8. gram stain purpose of each reagent #3
    the decolorizer remives the crystal violet from the gram negative bacteria cell wall, but not the gram possitive.
  9. gram stain purpose of each reagent #4
    the secondary stain safrain counterstains the gram negative bacteria with a red or pink color
  10. gram stain procedure
    step#1
    • transfer a loop of stock culture to a slide.
    • air dry.
    • heat fix and place on a rack.
  11. gram stain procedure step#2
    • flood slide with crystal violet for one minute.
    • rinse slide with water and place back on rack.
  12. gram stain procedure step#3
    • flood slide with iodine for one minute.
    • rinse with water.
  13. gram stain procedure step#4
    • drip decolorizer onto the slide and let it run off for 5 seconds
    • rinse with water and place back on rack
  14. gram stain procedure step#5
    • flood slide with saffranin for one minute.
    • rinse with water and blot dry.
    • observe under the microscope
  15. kinyoun acid-fast staining proceedure step #1
    • transfer stock culture to slide.
    • air dry. heat fix.
    • place slide on rack and flood with carbofuschsin for 5 min.
    • rinse with water.
  16. kinyoun acid-fast staining proceedure step#2
    • drip decolorizer on slide to rinse the stain from the specimen, until there is just a tint of color running off.
    • rinse with water.
  17. kinyoun acid-fast staining proceedure step #3
    • flood with brillant green stain for 2 min.
    • rinse with water, blot dry.
    • observe under microscope.
  18. kinyoun acid-fast staining reagents step #1
    primary stain, carbon fusion, is retained by the acid fast waxy complex in the cell wall
  19. kinyoun acid-fast staining reagents step #2
    the decolorizer removes the carbonfuschsin from the non-acid fast bacteria.
  20. kinyoun acid-fast staining reagents step #3
    the counter stain, brilliant green, stains the non-acid fast bacteria a greenish- blue
  21. 2O2 superoxide equation
    • 2O-2+2H===>O2+H2O2
    • superoxide/dismutase= oxygen + hydrogen peroxide
    • 2H2O2===> 2H2O+O2
    • catalaseor/peroxidase= water + free oxygen
  22. "OF" proceedure materials
    • 2 "OF" tubes,
    • needles,
    • stock cultures,
    • mineral oil.
  23. How to perform an "OF" proceedure.
    • using needles innoculate both tubes of "OF" with stock culture by stabbing each tube 3-4 times, but not all the way to the bottom.
    • Overlay one tube with mineral oil, leave both caps loose.
    • incubate overnight at 35 degrees
    • leave them all open.

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