Micro Lab 9
Card Set Information
Micro Lab 9
micro lab 9 study guide
Describe how to perform the Kinyoun (cold method) acid-fast staining procedure, step by step, and the purpose of each reagent used.
Transfer stock culture to slide, air dry, heat fix
Place slide on rack and flood with carbofusion for 5 min, rinse with water & drip decolorizer on slide to rinse until there is just a tint of color running off.
Rinse with water place on rack and flood with brilliant green for 2 min.
Rinse with water, blot dry, and observe under microscope
: carbofusion is retained by acid fast waxy complex in cell wall.
: removes primary from non-acid fast bacteria
: stains the non-acid fast bacteria blue green or green
What is the major difference between the Zieh-Neelson and the Kinyoun acid-fast stain procedure? What is in the Kinyoun carbofuschsin that is not in the Zieh-Neelsen's and what does it do?
Zieh-Neelson uses heat to penetrate cell wall with carbofusion
Kinyoun- Uses a wetting agent instead of heat to get the stain into the cell wall with its high lipid content
Carbofusion in Kinyoun had torbitity 7 or 4 as a wetting agent
Why are some organisms acid-fast and list 3 organisms that are?
High lipid in the cell wall.
: Microbacterium, norcardia, cryptosporidium
Can Gram positive or Gram negative organisms be acid-fast?
No, because these bacteria do not contain the high lipid content in the cell wall that is necessary to retain the acid-fast stain upon decolorization