Where should we meet if the lab room needs to be evacuated in an emergency?
D.
When there is an accident in the lab, which phrase should be said loudly to alert the instructor?
B.
What items MUST be worn at all times while working with bacteria in the lab?
B.
What two parts of the microscope are used to adjust the amount of light going through a specimen?
A.
The proper time to put immersion oil on a slide is after focusing which objective lens?
D.
How should the striker (sparker) be held to the top of the burner as it is being ignited?
C.
The hottest part of a Bunsen burner flame is located
A.
TRUE/FALSE: As the power of the objective lenses being used increases, the amount of light going throught the specimen should also increase.
False
In a properly done isolation streak, how may times are the bacteria actually spread over the agar surface?
D.
Which of the following compounds is the substrate for the catalase enzyme?
B.
Which of the following would be a positive test result in the slide coagulase test?
D.
Which would be a positive result for the presence of the catalase enzyme in a bacterium?
B.
TRUE/FALSE: Between transferring two different types of bacteria with an inoculating loop, you should put only the tip of the loop in the burner flame.
False
Having performed the Phenol Red broth test for a certain bacterium, you notice that the broth tube is yellow without gas in the Durham tube. What result would you record in your lab notes?
A.
Having performed the Phenol Red broth test for a certain bacterium, you notice that the broth tube is yellow and it has gas in the Durham tube. What result would you record in your lab notes?
B.
Having performed the Phenol Red broth test for a certain bacterium, you notice that the broth tube is pink without gas in the Durham tube. What result would you record in your lab notes?
D.
Having inoculated a tube of O-F medium with a certain bacterium, you left it unsealed. After incubation, it has a slight amount of yellow at the top. what symbol would you record in your lab notes?
B.
Having inoculated a tube of O-F medium with a certain bacterium, you left it sealed with mineral oil. After incubation, it is green throughout. What symbol would you record in your lab notes?
B.
You have inoculated a certain bacterium into fluid thioglycollate medium. After incubation, there is a cloudy section indicating growth only in the top centimeter. What is the aerotolerance of this organism? a. none of these
b. facultative anaerobe
c. obligate aerobe
d. strict anaerobe
e. microaerophile
c.
You have inoculated a certain bacterium into fluid thioglycollate medium. After incubation, the top centimeter is clear, but there is a cloudy section indicating growth everywhere else in the tube. What is the aerotolerance of this organism? a. obligate aerobe
b. none of these
c. facultative anaerobe
d. microaerophile
e. strict anaerobe
e.
You have inoculated a certain bacterium into fluid thioglycollate medium. After incubation, there is a thin cloudy section indicating growth only in the second centimeter down from the top. What is the aerotolerance of this organism? a. microaerophile
b. obligate aerobe
c. none of these
d. facultative anaerobe
e. strict anaerobe
a.
Your uninoculated control tube of fluid thioglycollate medium, having been incubated, shows a distinct pink coloration in the top centimeter of the medium. What does this indicate? a. contamination of the medium
b. diffusion of oxygen into the medium
c. two different bacteria were added to the medium
d. reduction of the medium
b.
After inoculating a bacterium onto a blood agar plate and incubating it for 24-48 hours, you notice there is a clear white zone around the bacteria. This demonstrates which type of hemolysis?
B.
After inoculating a bacterium onto a blood agar plate and incubating it for 24-48 hours, you notice there is a greenish zone around the bacteria. This demonstrates which type of hemolysis?
C.
After inoculating a certain bacterium onto a blood agar plate and incubating it for 24-48 hours, you notice there is no change in the color or appearance of the agar next to the bacteria. This demonstrates which type of hemolysis?
C.
In mannitol salt agar, the mannitol acts as...
D.
The agar that contains two dyes which select against the growth of Gram-positive organisms is...
D.
Bacteria that can ferment lactose when inoculated onto MacConkey agar would appear...
D.
If you want to differentiate between pathogenic and non-pathogenic staphylococci, the best agar to use is
D.
You have two different unknown bacteria. They both Gram stained as Gram+ staphylococcal species. You perform a coagulase test because no other media are available. The liquid smear for the first organism shows clear clumping of the bacteria. What is it most likely to be?
a. S. aureus b. B. cereus c. E. coli d. S. epidermis e. E. faecalis
a.
While trying to determine a bacterial unknkown, you find that it is a Gram- rod which produces a green sheen when cultured on EMB agar. Which of these organisms is your unknown?
a. Enterobacter aerogenes b. Staphylococcus aureus c. Staphylococcus epidermidis d. Escherichia coli
d.
You are working to identify a second bacterial unknown. So far, you have determined that it is a Gram+ coccus which is coagulase positive and creates a yellow halo when cultured on a mannitol salt agar. Which of these organisms is the unknown?
a. Enterobacter aerogenes b. Eschericia coli c. Staphylococcus epidermis d. Staphylococcus aureus
