Viro 2/E1

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Author:
HLW
ID:
98459
Filename:
Viro 2/E1
Updated:
2011-08-27 15:15:51
Tags:
Viro
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Description:
Viro 2/E1
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  1. TCID50-
    50% Tissue Culture Infectious Dose
  2. ID50-
    50% Infectious Dose
  3. LD50-
    Lethal Dose
  4. MOI-
    • Multiplicity of Infection
    • Number of Virus Particle provided for Each Cell
  5. Virus infection of cultured cells appears as…
    • General cell death
    • Plaque formation
    • Syncytial formation
    • Formation of inclusion bodies
    • Cell transformation
    • Persistent infection
  6. Cell damage by viruses
    • Accumulation of viral structural components
    • Formation of virion aggregates within the cell
    • Shutdown of cellular protein synthesis
  7. Isolation is utilized for…
    Immunological Detection
  8. Quatitation is utilized for…
    • TCID50
    • Plaque Assay
  9. Which Test is frequently used in the lab and in clinics
    ELISA
  10. Types of Virus Titration:
    • Plaque
    • Pock
    • Transformation
    • Hemaglutination/HI
  11. What tests are used to determine TCID50?
    • Plaque
    • Pock
    • Transformation
    • (Titration)
  12. Bovine Herpes Virus can be detected using…
    • ELISA
    • Immunofluorescence
    • Immunohistochemistry
  13. What Viruses are often tested in clinics, using ELISA(8)?
    • FeLV
    • FIV
    • Parvovirus
    • Bovine Herpes virus
    • Bovine viral diarrhea virus
    • Equine Infectious Anemia virus
    • Influenza-A
    • Porcine Respiratory Reproductive Syndrome Virus
  14. What Tests Can Detect Viral Nucleic Acids?
    • Hybridization
    • PCR
    • Nucleic Sequencing
  15. Methods for Virus Isolation
    • Cell Culture
    • Animal Inoculation
    • Embryonated Egg Inoculation
  16. What Methods are used to ID viral isolates?
    • Virusneutralization
    • Hemagglutination/Inhibition
    • Hemadsorption and hemadsorption inhibition
    • Complementfixation
    • Immunodiffusion
    • Restriction endonuclease emapping
    • Radiolabeling of Protein and Immunoprecipitation
    • Western Blot Analysis
  17. +HG=
    No button
  18. +HAI=
    Button
  19. Complement Fixation Test=> Lysis of RBC’s. This indicates that…
    No complement
  20. Methods Used to Detect exposure (3)
    • (Serology)
    • Virus neutralization
    • Hemagglutination inhibition
    • ELISA
  21. Limitations of serology
    • Detects exposure not when exposure occurred
    • For correlation with disease
    • Paired sera
    • IgM
    • CSF

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